牡丹江市朝鲜族人群15个常染色体STR基因座的频率调查

目的：调查牡丹江市朝鲜族人群无关个体的15个常染色体STR基因座（D2S1338、D18S51、D7S820、D8S1179、D5S818、D13S317、D16S539、vWA、D19S433、TPOX、D3S1358、FGA、CSF1PO、D21S11、TH01）的多态性,研究其在法医学亲子鉴定和个人识别检验中的实际应用价值。方法：应用AmpFL STR IdentifilerTM试剂盒扩增牡丹江市500例朝鲜族无血缘关系个体的15个STR基因座,其产物用ABI 310遗传分析仪进行检测,包括电泳分离和分型检测,分析其基因频率和遗传平衡分析,并计算分析其遗传参数：杂合度（H）、多态性信息含量（PIC）、个人识别能力（DP）。结果：500名无血缘关系个体各基因座基因型分布均符合Hardy-Weinberg平衡（P〉0.05）。15个STR基因座的H都大于0.6,DP都大于0.8,PIC都大于0.5。结论：15个STR基因座在牡丹江市朝鲜族人群中等位基因分布较好,具有较高的遗传多态性,所得的群体遗传学数据可满足该地区人类遗传学、法医学亲子鉴定和个人识别等领域的研究需要。

A GENETIC POLYMORPHISM OF 15 STR LOCI IN CHAOXIAN POPULATION OF MUDANJIANG

Objective：To investigate the genetic polymorphism of 15 short tandem repeat （STR） loci （D2S1338 ,D18S51 ,D7S820, D8S1179 ,D5S818 ,D13S317 ,D16S539 ,vWA ,D19S433 ,TPOX ,D3S1358 ,FGA,CSF1PO ,D21 S11 ,TH01 ） in Korean population of Mu- danjiag and study the actual value of forensic medicine paternity test and personal identification. Methods： Using the AmpFL STR Iden- tifilerTM PCR Amplification and 310 DN,~ Genetic Analyzer to detect the allelic freqyencies of 15 STR loci in 500 unrelated individuals of Korean population of Mudanjiang and test genetic balance of 15 STR loci. Heterozygosity （ H）, polymorphic information content （PIC） ,discrimination power（DP） were calculated. Results：The distribution of genotype frequencise of the 15 STR loci met Hardy- Weinberg balance（ P 〉 0.05 ）. H 〉 0.6 of 15 STR loci, DP 〉 0.8, PIC 〉 0.5. Conclusion： All of the 15 STR loci in Korean popula- tion of Mudanjiang have a better distribution and higher genetic polymorphisms, and can be used as the genetic markers of Korean pop- ulation in .the research of human genetics, paternity test and personal identification in forecsic medicine in this area.