TWEAK在肝癌细胞系高/低淋巴道转移株中表达的初步分析

目的]研究肿瘤坏死因子样弱凋亡因子（TWEAK）在小鼠腹水型肝癌细胞系高／低淋巴道转移株（Hca—F／Hca—P）中的表达及差异。方法]分别提取Hca—F和Hca—P细胞的总RNA，通过RT—PCR在mRNA水平检测TWEAK基因的表达；提取Hca—F和Hca—P细胞的总蛋白，用蛋白印迹法从蛋白表达水平验证差异表达。结果]RT—PCR检测到两株细胞均在358bo处出现特异性扩增的条带，并且Hca—F的条带亮度低于Hca—P，图像分析显示，Hca—F细胞中TWEAK的转录子含量低于Hca—P细胞（P〈0．05）；蛋白免疫印迹法验证两株细胞表面均有TWEAK蛋白的表达，Hca—P强于Hca—F，与基因芯片的结果一致。结论]TWEAK在鼠腹水型肝癌细胞系Hca—F和Hca—P均有表达，且Hca—F中较低，而在Hca—P中表达较高。这说明了TWEAK可能与淋巴道转移有关，同时可能参与了细胞的凋亡与组织分化。

Expression of TWEAK in mouse hepatocarcinomacell lines Hca-F and Hca-P

Objective] To study the expression difference of TWEAK in Hca-F and Hca-P cell lines.Methods] Total RNA was extracted from the two cells lines(Hca-F and Hca-P),RT-PCR was carried out to verify the expression of TWEAK.For further study,Western blot was performed to confirmed the TWEAK expression with the total protein from the two cell lines.Results] The expression of TWEAK mRNA and protein in Hca-P cells were higher than that in Hca-F cells,as revealed by RT-PCR,Western blot.Conclusion] The expression of TWEAK mRNA and protein in Hca-P cells were higher than that in Hca-F cells,and our study may facilitate the understanding of mechanism for tumor lymph node metastasis,the TWEAK may be the new target for anti-tumor therapy.