| 16SrRNA-PCR在淋球菌引起的泌尿生殖道感染检测中的应用 |
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| 引用本文： | 谢良伊,蔡瑞云.16SrRNA-PCR在淋球菌引起的泌尿生殖道感染检测中的应用[J].实用预防医学,2010,17(3):571-573. |
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| 作者姓名： | 谢良伊 蔡瑞云 |
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| 作者单位： | 湖南省人民医院检验科,湖南,长沙,410005 |
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| 摘 要： | 目的建立一种快速、特异性、敏感地检测淋球菌致泌尿生殖道感染的16SrRNA-PCR基因诊断方法,以了解泌尿生殖道感染中淋球菌的感染情况。方法以淋球菌16S核糖体rRNA基因为扩增靶点,设计一对特异性的引物扩增靶基因片段,扩增的片段长度为260bp,扩增产物通过ABI3130 DNA Analyzer进行测序,测序结果同Genebank报道的序列进行比较,评价该方法的特异性;对淋球菌模板进行10倍比稀释,进行16SrRNA-PCR,对其敏感性进行分析,对PCR扩增产物进行电泳和测序鉴定。结果泌尿生殖道感染中通过16SrRNA-PCR扩增可检出淋球菌16SrRNA基因,通过序列分析证实了PCR产物的特异性;在敏感性检测中16SrRNA-PCR检测淋球菌模板的下限为6.41×10-4μg/ml。结论本研究建立的16SrRNA-PCR法检测泌尿生殖道淋球菌感染具有简便、特异、快速的特点,可作为临床上女性宫颈分泌物及尿液标本的检测。
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| 关 键 词： | 16SrRNA-PCR 淋球菌感染 基因测序 |
Utilization of 16SrRNA-PCR in Detection of Genitourinary Tract Infection Caused by Neisseria Gonorrhoeae |
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| XIE Liang-yi,CAI Rui-yun.Utilization of 16SrRNA-PCR in Detection of Genitourinary Tract Infection Caused by Neisseria Gonorrhoeae[J].Practical Preventive Medicine,2010,17(3):571-573. |
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| Authors: | XIE Liang-yi CAI Rui-yun |
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| Institution: | XIE Liang-yi,CAI Rui-yun (Department of Laboratory Medicine,Hunan Provincial People\'s Hospital,Changsha 410005,Hunan,China) |
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| Abstract: | Objective To establish a rapid,specific and sensitive polymerase chain reaction (16SrRNA-PCR) assay for the detection of genitourinary tract infection caused by Neisseria gonorrhoeae,and to know the prevalence of Neisseria gonorrhoeae in genitourinary tract infection. Methods According to the sequences of Neisseria gonorrhoeae 16SrRNA,a couple of specific primers were designed to amplify the target gene sequence,and the size of the amplified fragment was 260 bp. The amplified products were analyzed by ABI31... |
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| Keywords: | 16SrRNA-PCR |
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