| 板蓝根颗粒中精氨酸的柱前衍生化HPLC测定 |
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| 引用本文： | 陈矛,曾令杰,陈松光. 板蓝根颗粒中精氨酸的柱前衍生化HPLC测定[J]. 现代中药研究与实践, 2003, 17(5): 42-44 |
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| 作者姓名： | 陈矛 曾令杰 陈松光 |
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| 作者单位： | 广州白云山制药股份有限公司,广东,广州,510515 |
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| 基金项目： | 广东省科技厅课题(JB-02) |
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| 摘 要： | 目的 建立一种定量分析方法评价板蓝根颗粒的质量。方法 柱前衍生化 HPL C法 ,以 Phenom enODS C1 8柱 (10μm,4 .6 mm× 2 5 0 mm )分析柱 ;0 .1m ol/ L醋酸钠缓冲液 (p H6 .4 ) -乙腈 (86∶ 14 )为流动相 ,流速 1.0 ml/ m in;36 0 nm下进行检测 ,分析了不同来源的板蓝根颗粒中精氨酸的含量。结果 通过该法 ,成功地测定了板蓝根颗粒中精氨酸的含量 ,并且发现 ,不同来源的板蓝根颗粒中精氨酸的含量差别很大。结论 该法操作简单 ,重复性好 ,准确可靠 ,可以作为板蓝根质量控制的一种方法
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| 关 键 词： | 板蓝根 柱前衍生化HPLC 精氨酸 |
| 文章编号： | 1004-2199(2003)05-0042-03 |
| 修稿时间： | 2003-08-15 |
Determination of Arginine in Banlangen Granule by a Pre-column Derivatization HPLC Method |
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| CHEN Mao,Zeng Ling-jie,CHEN Song-guang. Determination of Arginine in Banlangen Granule by a Pre-column Derivatization HPLC Method[J]. Research and Practice on Chinese Medicines, 2003, 17(5): 42-44 |
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| Authors: | CHEN Mao Zeng Ling-jie CHEN Song-guang |
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| Abstract: | Objective To establish a quantitative method for the quality control of Banlangen Granule.Method A pre-column derivatization HPLC method, in which the analyte was eluted in a phenomen ODS C 18 column (10 μm, 4.6 mm×250 mm) with 0.1 mol/L NaAc buffer (pH6.4) -acetonitrile (86∶14) in the flow rate of 1.0 ml/min, was applied to analyze the arginine content of different factories under the wave length of 360 nm. Results Arginine content in Banlangen Granule was successfully determined, and it was found that the content of arginine in Banlangen Granule from different sources varied greatly.Conclusions The method is simple, reproducible and accurate. It can used as a quality control method for Banlangen Granule. |
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| Keywords: | Isatis indigotica Fort. pre-column derivatization HPLC arginine |
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