氟烷性肝炎中抗体依赖细胞介导的细胞毒作用对肝细胞的毒性

目的：探讨氟烷性肝炎免疫应答中抗体依赖细胞介导的细胞毒作用（ADCC）对肝细胞的毒性作用。方法：雄性Hartley豚鼠在40％O2下吸入1％氟烷4h，诱导1次，或按分组需要间隔42d后同上再吸入1次，再隔42d后同上再吸入I次，诱导3次。对照组只吸入40％O2。分离淋巴细胞和肝Kupffer细胞，两种细胞按一定的分类和比例混合培养，再加入含有特异性抗体和细胞因子的上清液，对照分析机体吸入氟烷后诱导免疫应答过程中，ADCC对肝细胞的毒性作用。结果；氟烷诱导3次豚鼠肝细胞毒性作用包括特异性抗体介导的ADCC作用，氟烷诱导1次豚鼠ADCC作用仍需氟烷对离体肝细胞的再次诱导。Kupffer细胞诱导淋巴细胞增生成的特异性抗体和分泌的细胞因子对肝细胞无毒性作用。结论：体液免疫主要是ADCC，单纯的特异性抗体和细胞因子不能杀伤肝细胞。免疫应答反应的强弱与吸入氟烷有关。

Antibody dependent cell-mediated cytotoxicity on hepatocytes during immune response in halothane induced hepatitis

Objective: To study antibody dependent cell-mediated cytotoxicity (ADCC) on hepatocytes during immune response in halothane hepatitis of guinea pigs. Methods: The model of halothane hepatitis of guinea pigs was used. Mixed lymphocyte hepatocytes were cultured and supernatants containing specific antigens and cytokines were added. The changes of hepatic function in immune response of halothane hepatitis were analyzed. Results: The cytotoxicity on hepatocytes induced by halothane 3 times included ADCC mediated by specific antigens. ADCC on hepatocytes induced by halothane once required the second induction. There was no cytotoxicity on hepatocytes of specific antigens and cytokines produced by proliferative lymphocytes induced by Kupffer cells. Conclusion: Humoral immunity is mainly ADCC. Specific antibodies and cytokines can not separately kill hepatocytes. The extent of immune response is related to induction by halothane.