rhBMP-2在诱导C3H10T1/2干细胞成骨中对MMP-13、Collagen-2 mRNA表达的影响

目的研究基质金属蛋白酶13(MMP13)、II型胶原(collagen2)在C3H10T1/2细胞中的相互关系,探讨rhBMP-2在诱导C3H10T1/2成骨的同时对MMP13以及Collagen2 mRNA表达水平的影响。方法取C3H10T1/2细胞加入rhBMP-2诱导成骨细胞的产生,Von Kossa染色观察细胞形态,同时在不同时间段抽取总RNA,利用Real-time PCR检测MMP13、Collagen2的表达情况。结果未加rhBMP-2的细胞中MMP13、Collagen2前10d表达呈负相关;而rhBMP-2处理后的细胞中MMP-13表达水平受抑制,且MMP13和collagen2前14d表达呈明显负相关。结论MMP-13和Collagen-2 mRNA表达水平具明显相关性,rhBMP-2在诱导成骨的同时,具有抑制MMP-13表达的作用。

The Effect of rhBMP-2 on the Expression of MMP13 and Collagen-2 in mRNA Level during Osteogenic Differentiation

Objective To investigate the interaction of metalloproteinase-13 (MMP-13) and type II collagen (Col-2) in C3H10T1/2 cells, and explore the effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on the expression of MMP13 and Col-2 in mRNA level during osteogenic differentiation induced by rhBMP-2. Methods rhBMP was utilized to induce osteoblastic differentiation of C3H10T1/2 cells. Total RNA was isolated at various time points to detect the expression of MMP13 and Col-2 by the method of quantitative Real-time PCR. Results There was a negative correlation between MMP-13 and Col-2 expression in C3H10T1/2 cells without rhBMP-2 treatment in the first 10 days. In the rhBMP-2 treated cells, however, expression of MMP-13 was inhibited and there was a negative correla-tion between MMP13 and Col-2 expression in the first 14 days. Conclusions There is a obvious correlation between the expression of MMP13 and Col-2. rhBMP-2 inhibits the expression of MMP13 while it induces osteogenic differentiation of C3H10T1/2 cells.