高原鼠L-精氨酸预处理与第一肝门阻断时肠道细菌易位

目的探讨高原鼠L-精氨酸预处理对第一肝门阻断时肝缺血再灌注损伤和肠道细菌易位有无保护作用。方法SD大鼠在高原环境饲养14天后分为假手术组(5只)及实验组(持续阻断30分钟及60分钟组,L-精氨酸预处理加持续阻断30分钟及60分钟,每组5只)。无菌条件下用无损伤血管夹阻断大鼠第一肝门建立肝脏缺血再灌注(I/R)模型,再灌注24小时后在无菌条件下分别取门静脉血、回肠系膜淋巴结进行肠道细菌培养。观察外源性L-精氨酸预处理对第一肝门阻断时肝缺血再灌注损伤与肠道细菌易位有无保护作用。结果假手术组未培养出细菌;持续阻断30分钟组2例、持续阻断60分钟组5例、外源性断60分钟组5例血液及淋巴结均培养出大肠埃希氏菌。与假手术组比较,差异非常显著;持续阻断60分钟组亦明显高于持续阻断30分钟组,便各预处理组与相同阻断时间组之间无差异。结论高原第一肝门阻断的时间及肝缺血再灌注损伤对肠道细菌易位有显著的影响,但外源性L-精氨酸预处理对高原第一肝门阻断时肝缺血再灌注损伤和肠道细菌易位无明显的保护作用。

THE RELATIONSHIP BETWEEN EXOGENOUS L-ARGIMINE PRECONDITIONING AND BACTERIAL TRANSLOCATION IN RATS CAUSED BY PORTAL TRAID CLAMPING AT HIGH ALTITUDE

Aim To evaluate the relationship between exogenous L-argimine preconditioning and bacterial translocation caused by portal traid champing in rats at high altitude.Methods SD rats alived 14 days at high altitude were randomly divided into five groups:a sham operation group,a 30 minunte portal triad champing group,a 60 minunte portal triad champing group,a exogenous L-argimine preconditioning and 60 minunte ortal triad clamping group.Samples both blood and ileal mesenteric lymph onde were cultured at 24 hours after reperfused.Results No bacteria was culuured out in sham operation group.Escherichia coli(E,coli)was cultrued out in 2 rats of 30 minunte portal triad champing group,2 rats of exogenous L-argimine preconditioning and 30 minunte portal triad champing group,5 rats of 60 minunte portal triad champing group,5 rats of exogenous L-argimine preconditioning and 60 minunte portal triad champing group.Conclusion Portal triad clamping duration and liver ischemia-reperfusion injury could affect the occurred of acterial translocation.The protective role of exogenous L-argimine at high altitude is uneffective.