| 氧化修饰低密度脂蛋白刺激血管内皮细胞表达VCAM-1的实验研究 |
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| 引用本文： | 杜怡峰,孙圣刚,童萼塘.氧化修饰低密度脂蛋白刺激血管内皮细胞表达VCAM-1的实验研究[J].脑与神经疾病杂志,2001,9(2):80-81. |
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| 作者姓名： | 杜怡峰 孙圣刚 童萼塘 |
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| 作者单位： | 1. 青岛医学院附属医院神经内科,
2. 同济医科大学附属协和医院神经内科 |
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| 摘 要： | 目的：探讨氧化修饰低密度脂蛋白（ＯＸ－ＬＤＬ）对血管内皮细胞表达血管细胞粘附分子－１（ＶＣＡＭ—１）的影响，以阐明其在动脉粥样硬化发生中的作用。方法：在血管内皮细胞的培养基中分别加入２５μｇ／ｍｌ的低密度脂蛋白（ＬＤＬ）和ＯＸ－ＬＤＬ，３7℃下温育 ２４小时，应用定量免疫细胞化学分析技术检测内皮细胞 ＶＣＡＭ－１蛋白的表达。结果：与对照组和ＬＤＬ组比较，ＯＸ－ＬＤＬ组内皮细胞ＶＣＡＭ－１蛋白的表达显著增加。结抡：ＯＸ－ＬＤＬ能够明显刺激内皮细胞ＶＣＡＭ－１的表达。
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| 关 键 词： | 内皮细胞 粘附分子 氧化修饰低密度脂蛋白 动脉粥样硬化 |
| 修稿时间： | 2000年10月16 |
The study on effects of oxidatively modified low density lipoprotein on the Expression of VCAM-1 In vascular endothelial |
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| DU Yi Feng,SUN Seng Gang,TONG E Tang.The study on effects of oxidatively modified low density lipoprotein on the Expression of VCAM-1 In vascular endothelial[J].Journal of Brain and Nervous Diseases,2001,9(2):80-81. |
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| Authors: | DU Yi Feng SUN Seng Gang TONG E Tang |
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| Abstract: | Objective: To examine whether Oxidatively modified low density lipoprotein (OX - LDL) in duce the expression of vascular cell adhesion molecule-1 (VCAM-1) Protein in cultured human umbilical vein endothelial cells (HUVECs), and to clarify what a role of OX-LDL play in atherogenesis. Methods: On the basis of the establishment of culture method for HUVECs, The quantitative immunocytochemstry was used to study the effects of OX-LDL on the expression of VCAM-1 protein in HUVECS. Results:after the smaples of HUVESCs were subjected to 25 pg/ml LIJL and OX-LDL for 24h, the expression of VCAM-1 protein in HUVECs exposed to OX-LDL was markedly stronger than those of LL)L and control. Conclusion: OX-LDL Could stimulat the expression of SCAM-1, Which might Play an improtant role in the atherogenesis. |
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| Keywords: | Endothelial cell Adhesion milecule Oxidized low density lipoprotein Atherosclerosis |
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