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氧化修饰低密度脂蛋白刺激血管内皮细胞表达VCAM-1的实验研究
引用本文:杜怡峰,孙圣刚,童萼塘. 氧化修饰低密度脂蛋白刺激血管内皮细胞表达VCAM-1的实验研究[J]. 脑与神经疾病杂志, 2001, 9(2): 80-81
作者姓名:杜怡峰  孙圣刚  童萼塘
作者单位:1. 青岛医学院附属医院神经内科,
2. 同济医科大学附属协和医院神经内科
摘    要:目的:探讨氧化修饰低密度脂蛋白(OX-LDL)对血管内皮细胞表达血管细胞粘附分子-1(VCAM—1)的影响,以阐明其在动脉粥样硬化发生中的作用。方法:在血管内皮细胞的培养基中分别加入25μg/ml的低密度脂蛋白(LDL)和OX-LDL,37℃下温育 24小时,应用定量免疫细胞化学分析技术检测内皮细胞 VCAM-1蛋白的表达。结果:与对照组和LDL组比较,OX-LDL组内皮细胞VCAM-1蛋白的表达显著增加。结抡:OX-LDL能够明显刺激内皮细胞VCAM-1的表达。

关 键 词:内皮细胞  粘附分子  氧化修饰低密度脂蛋白  动脉粥样硬化
修稿时间:2000-10-16

The study on effects of oxidatively modified low density lipoprotein on the Expression of VCAM-1 In vascular endothelial
DU Yi Feng,SUN Seng Gang,TONG E Tang. The study on effects of oxidatively modified low density lipoprotein on the Expression of VCAM-1 In vascular endothelial[J]. Journal of Brain and Nervous Diseases, 2001, 9(2): 80-81
Authors:DU Yi Feng  SUN Seng Gang  TONG E Tang
Abstract:Objective: To examine whether Oxidatively modified low density lipoprotein (OX - LDL) in duce the expression of vascular cell adhesion molecule-1 (VCAM-1) Protein in cultured human umbilical vein endothelial cells (HUVECs), and to clarify what a role of OX-LDL play in atherogenesis. Methods: On the basis of the establishment of culture method for HUVECs, The quantitative immunocytochemstry was used to study the effects of OX-LDL on the expression of VCAM-1 protein in HUVECS. Results:after the smaples of HUVESCs were subjected to 25 pg/ml LIJL and OX-LDL for 24h, the expression of VCAM-1 protein in HUVECs exposed to OX-LDL was markedly stronger than those of LL)L and control. Conclusion: OX-LDL Could stimulat the expression of SCAM-1, Which might Play an improtant role in the atherogenesis.
Keywords:Endothelial cell Adhesion milecule Oxidized low density lipoprotein Atherosclerosis
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