Increased expression of alpha-enolase in cervico-vaginal fluid during labour |
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Authors: | Nicole L Dellios I Ross Young Jonathan J Hirst Mustafa Ayhan |
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Institution: | a Translational Proteomics, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria 3004, Australia b Department of Obstetrics and Gynaecology, University of Melbourne, Victoria 3084, Australia c Mercy Perinatal Research Centre, Mercy Hospital for Women, Victoria 3084, Australia d Department of Physiology, Monash University, Victoria 3800, Australia e Mothers and Babies Research Centre, University of Newcastle, New South Wales 2308, Australia f Proteomics, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria 3004, Australia |
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Abstract: | ObjectivesThe aim of this study was (i) to characterise differentially expressed proteins in cervico-vaginal fluid (CVF) at the time of preterm labour onset and (ii) to confirm these studies in human CVF samples taken from women before and during spontaneous labour.Study designPreterm labour was induced in sheep (n = 5) via fetal dexamethasone infusion (1 mg/24 h). CVF samples were taken prior to dexamethasone infusion (0 h), 28 h after the start of dexamethasone infusion, and immediately prior to delivery. Two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to identify differentially expressed proteins. For the human studies, paired CVF samples were taken 5-9 days before labour and during spontaneous labour onset (n = 7).ResultsThere was a 4.2-fold increase in α-enolase protein expression in sheep CVF during labour. Likewise, α-enolase protein expression was significantly increased during spontaneous human labour at term.ConclusionsAlpha-enolase is known to be bound to neutrophils and interact in the immune response, and thus may play a role in inflammation associated with human labour. |
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Keywords: | Ovine Human labour Cervico-vaginal fluid Alpha-enolase Two-dimensional gel electrophoresis Mass spectrometry |
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