| 罗红霉素对哮喘大鼠气道重塑中成肌纤维细胞、TGF-β_1和IFN-γ的影响 |
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| 引用本文： | 林洁,戴元荣,赵初环,翁海霞,夏晓东,何剑波.罗红霉素对哮喘大鼠气道重塑中成肌纤维细胞、TGF-β_1和IFN-γ的影响[J].中国临床药理学与治疗学,2008,13(10). |
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| 作者姓名： | 林洁 戴元荣 赵初环 翁海霞 夏晓东 何剑波 |
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| 作者单位： | 1. 温州医学院附属第二医院呼吸内科,温州,325027,浙江
2. 温州医学院附属第一医院心脏监护室,温州,325000,浙江 |
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| 基金项目： | 浙江省科技厅资助基金，温州市科技局资助基金 |
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| 摘 要： | 目的:探讨成肌纤维细胞(myofibroblast,MF)、转化生长因子β1(transforming growth factor-be-ta1,TGF-β1)和IFN-γ在哮喘气道重塑中的作用,并观察罗红霉素对哮喘气道重塑的影响。方法:SD大鼠30只,随机分为哮喘组、生理盐水对照组、罗红霉素治疗组,每组10只。利用卵白蛋白(ovalbumin,OVA)/Al(OH)3致敏与OVA雾化吸入激发建立大鼠哮喘模型。免疫组化测定支气管上皮下成肌纤维细胞的α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)表达含量,并使用图像分析技术进行积分吸光度(integral optical density,IOD)定量分析测定。ELISA法测定支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中TGF-β1和IFN-γ的浓度。结果:定量分析测定的IOD值显示哮喘组支气管上皮下成肌纤维细胞α-SMA表达含量较生理盐水对照组增加(P<0.01),罗红霉素治疗组表达含量较哮喘组减少(P<0.05)。ELISA法测定哮喘组BALF中TGF-β1浓度较对照组升高(P<0.01...
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| 关 键 词： | 哮喘 气道重塑 成肌纤维细胞 罗红霉素 |
Effects of roxithromycin on myofibroblast,TGF-β1 and IFN-γin rats asthma airway remodeling |
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| LIN Jie,DAI Yuan-tong,ZHAO Chu-huan,WENG Hai-xia,XIA Xiao-dong,HE Jian-bo.Effects of roxithromycin on myofibroblast,TGF-β1 and IFN-γin rats asthma airway remodeling[J].Chinese Journal of Clinical Pharmacology and Therapeutics,2008,13(10). |
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| Authors: | LIN Jie DAI Yuan-tong ZHAO Chu-huan WENG Hai-xia XIA Xiao-dong HE Jian-bo |
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| Institution: | 1Department of Pulmonology; the Second Affiliated Hospital; Wenzhou Medical College; Wenzhou 325027; Zhejiang; China; 2Department of Cardiac Care Unit; the First Affiliated Hospital; Wenzhou 325000; |
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| Abstract: | AIM:To explore the effects of myofibroblast,TGF-β1 and IFN-γ on asthma airwy remodeling and observe the influence of roxithromycin on asthma airway remodeling.METHODS:In the experiment,thirty SD rats were randomly divided into the asthma group(group A,n=10),the sodium chloride control group(group C,n=10) and the roxithromycin group(group R,n=10).The rats were sensitized and stimulated with ovalbumin/Al(OH)3 and OVA atomization inhalation respectively to establish the asthma model.The α-smooth muscle actin(α... |
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| Keywords: | asthma airway remodeling myofibroblast roxithromycin |
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