抗-A和抗-B单克隆抗体对红细胞内血红蛋白释放情况的影响

目的:探讨抗-A、抗-B单克隆抗体对红细胞内血红蛋白电泳释放的影响。方法:用A、B、O、AB 4种血型红细胞与抗-A及抗-B单克隆抗体反应后的产物进行淀粉-琼脂粉混合凝胶电泳,常规电泳后停电30 min再继续电泳,观察血红蛋白的释放情况。结果:A、B、O、AB型红细胞与单克隆抗体反应后血红蛋白释放情况不同,与此同时HbA2的电泳行为也发生变化。结论:单克隆抗体对各型红细胞的血红蛋白释放有影响。

The Effect of Anti-A and Anti-B Monoclonal Antibody on Erythrocytic Hemoglobin Release

Objective： To evaluate the effect of Anti - A and Anti - B monoclonal antibody on erythrocytic hemoglobin elec- trophoresis release. Methods： Reaction products of erythrocytes from blood group A, B, O and AB with their corresponding mon- oclonal antibodies underwent starch - agarose mixed gel electrophoresis, with electrophoresis resumed after a interruption of 30 minutes, and the hemoglobin release from ery throcytes was observed. Results： Erythrocytes in blood group A, B, O and AB changed their hemoglobin release manners upon reacting with their corresponding monoclonal antibodies and the electrophoresis behaviors of HbA2 of these erythrocytes changed as well. Conclusion： Hemoglobin release from erythrocytes is affected by their reaction with monoclonal antibodies. The research established an important approach for the further investigation of the release mechanism.