IL-11在半相合混合骨髓移植造血恢复中作用的研究

目的 观察白介素-11 IL-11在活化半相合混合骨髓移植小鼠造血恢复中的作用。方法 以急性放射病615小鼠模型为受鼠,615×C57BL/6杂交F1代小鼠为半相合供鼠。半相合鼠骨髓和脾细胞中混合一定比例的同基因脾细胞,进行半相合混合骨髓移植。观察移植后小鼠死亡率、白细胞系造血重建、骨髓集落形成、脾结节及病理学改变。结果 加用IL-11的半相合混合骨髓移植组(供受体小鼠脾细胞比例2:1)为外周血白细胞及骨髓集落数量明显高于未加用IL-11组。结论 IL-11可以明显促进半相合混合骨髓移植小鼠造血恢复。

Effect of IL-11 on Haematopoisis in Mice with Half-matched Mixed Bone Tarrow Rransplantation

Objective To observe effect of IL-11 on hematopoisis in mice with half-matched mixed BMT.Methods Mice 615 with acute radiation disease were used as recipient mice model, with 615xC57BL/6 hybrid F1 mice being half-matched donor mice.Half-matched murine bone marrow and spleen cells mixed with certain percent of syngeneic spleen cells (2:1) and underwent mixed half-matched BMT to observe their death rate, hematopoietic recovery of WBC lineage, GM-CFU, spleen node count and pathological changes.Results The half-matched mixed 2:1 BMT group with high percentage of syngeneic spleen cells could lessen the extent of GVHD, as manifested by the quick recovery of peripheral blood WBC count and bone marrow GM-CFU formation.Conclusion IL-11 could promote the recovery of hematopoisis in half-matched mixed BMT mice.