Effects of chronic ethanol exposure on cultured cerebellar granule cells |
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| Authors: | Fausta Omodeo-Salé Marina Pitto Massimo Masserini Paola Palestini |
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| Affiliation: | 1. Institute of General Physiology and Biochemistry, Faculty of Pharmacy, University of Milan, Italy
2. Department of Medical Chemistry and Biochemistry, The Medical School, University of Milan, Italy
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| Abstract: | The aim of this study was to investigate the lipid content and composition of rat cerebellar granule cells grown in the presence of ethanol (40, 55, or 80 mM) during in vitro differentiation. Quantitative analyses showed no effects of 40 mM ethanol, whereas a significant increase of total cholesterol was observed at 55 mM. Cells exposed to the highest ethanol dose (80 mM) were characterized by a higher sialidase activity, and by the modification of the ganglioside pattern and phospholipid fatty acid composition. The observed modifications were accompanied by changes of membrane anisotropy fluorescence assessed by the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene. |
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