| Babesia Microti感染鼠对Babesia Rodhaini再感染交叉免疫保护机理的研究 |
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| 引用本文： | 刘云,王洪斌,小野宪一郎. Babesia Microti感染鼠对Babesia Rodhaini再感染交叉免疫保护机理的研究[J]. 免疫学杂志, 2008, 24(6): 656-659,662 |
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| 作者姓名： | 刘云 王洪斌 小野宪一郎 |
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| 作者单位： | 东北农业大学动物医学学院临床医学系;东京大学农学部大学院; |
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| 摘 要： | 目的研究Babesia Microti和Babesia Rodhaini感染的交叉免疫保护作用,以探讨B.Microti和B.Rodhaini不同种属之间是否存在交叉保护及保护机制。方法首先制备Babesia Microti感染耐过鼠,再用Babesia Rodhaini进行再感染,分别于B.Rodhaini再感染后的12、24、48、72h,使用ELISA方法分别检测血清中IL-12及IL-10的浓度,并同时检测寄生虫血症、网织红细胞变化、致死率与死亡时间。结果B.Microti感染耐过鼠再感染Babesia Rodhain后24h,小鼠血液中IL-12较未感染组及B.Microti感染耐过鼠明显增高,而血液中IL-10水平在再感染后72h时与对照组比较差异显著。B.Microti感染耐过鼠再感染Babesia Rodhain后,血液中出现一过性寄生虫血症,实验鼠可完全耐过。结论B.Microti感染耐过鼠可完全保护B.Rodhain的再感染,其细胞因子的产生为IL-12早于IL-10。
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| 关 键 词： | B.Microti B.Rodhaini IL-12 IL-10 |
Cross protection in Babesia Microti immunized mice against Babesia Rodhaini reinfection |
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| LIU Yun,WANG Hong-bin,KENICHIRO Ono. Cross protection in Babesia Microti immunized mice against Babesia Rodhaini reinfection[J]. Immunological Journal, 2008, 24(6): 656-659,662 |
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| Authors: | LIU Yun WANG Hong-bin KENICHIRO Ono |
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| Affiliation: | Department of Clinical Medicine;College of Veterinary Medicine;Northeast Agricultural University;Harbin 150030;China |
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| Abstract: | Objective To study the cross protection in Babesia Microti immunized mice against Babesia Rodhaini reinfection and to understand whether there is any protection between different species (B. Microti and B. Rodhaini) Methods A total of 40 Balb/c mice,recovered from B. Microti infection,were reinfected with B. Rodhaini. The sera of the mice were collected at 12,24,48,and 72 h after the reinfection for detecting IL-12 and IL-10 levels using ELISA. The parasitemia,reticulocytes,mortality,and the death time of t... |
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| Keywords: | B.Microti B.Rodhaini IL-12 IL-10 |
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