马齿苋活性成分体内外抗癌作用的初步筛选

目的 观察马齿苋活性成分对人肺腺癌细胞系(A-549 cell)、人喉表皮样癌细胞系(Hep-2 cell)、人宫颈癌细胞系(Hela cell)和人恶性胚胎横纹肌瘤细胞系(RD cell)生长的影响.方法 体外培养条件下用不同浓度的马齿苋活性成分处理4种癌细胞,通过溴化二甲噻唑二苯四氮唑(MTT)试验法测定癌细胞的增殖;同时处理S180荷瘤小鼠,观察荷瘤小鼠的体重、瘤重、死亡率和抑瘤率.结果 马齿苋生物碱对离体培养的A-549肺癌细胞、Hela细胞和Hep-2细胞的增殖均具有明显抑制作用,马齿苋多糖对Hela细胞有较强的抑制作用, 马齿苋脂肪酸对Hep-2细胞有一定的抑制作用,马齿苋黄酮对RD细胞有很强的抑制作用,并存在浓度剂量效应,高浓度抑制作用强.结论 马齿苋活性成分能选择性地杀伤癌细胞,有进一步研究的潜在价值.

Screening Antitumor Effect of Active Constituents from Portulaca oleracea L. in vitro and in vivo

Objective To observe the effects of the active constituents from Portidaca oleracea L. on the proliferation of four human tumor cell lines. Methods The anti - proliferation activities of active constituents from Portidaca oleracea L. upon A549 cell, Hela cells, Hep -2 cell and RD cell were analyzed by MTT in vitro. Body weigt, tumor weigt, inhibition rate and death rate of these active constituents from Portidaca oleracea L. on the transplanted tumor cells of Sarcinoma 180 were observed in mice. Results Raw screening of the four compounds by MTT indicated polysaccharides, flavonoids, alkaloids and polyunsaturated fatty acid exerted obvious suppression on the proliferation of tumor cell lines in a dose dependent manner, respectively. The experiments in vivo indicated that the four active constituents had certain inhibition against the cells of implanted tumor ascites Sarcinoma 180. Conclusion The active constituents from Portidaca oleracea L. have selective cytotoxicity to the tumor cells and is worthy of further investigation.