葛根总黄酮对载脂蛋白E基因缺陷小鼠动脉粥样硬化斑块形成的影响

目的探讨葛根总黄酮对载脂蛋白E基因缺陷小鼠主动脉窦动脉粥样硬化斑块形成的影响。方法将载脂蛋白E基因缺陷小鼠随机分为模型组、葛根总黄酮低剂量组、葛根总黄酮高剂量组及阿托伐他汀阳性药物组。常规生物化学法测定血清总胆固醇、甘油三酯及高密度脂蛋白胆固醇水平;采用主动脉根部连续石蜡切片、苏木素伊红染色观察组织形态学改变,测定载脂蛋白E基因缺陷小鼠主动脉窦动脉粥样硬化斑块大小、动脉粥样硬化斑块与管腔面积比。结果4组小鼠共检出斑块65个,4组虽都有典型的粥样斑块或/及纤维斑块等中晚期动脉粥样硬化病变形成,但组织学观察发现模型组部分斑块体积较大,汇合成片,管壁弥漫性增厚,管腔较狭窄,而葛根总黄酮治疗组及阿托伐他汀阳性药物组动脉粥样硬化斑块病变较局限,管腔狭窄程度较轻,并且葛根总黄酮高、低剂量组及阿托伐他汀阳性药物组斑块面积与管腔面积之比值显著低于模型组(分别为0.11%±0.04%、0.27%±0.04%和0.76%±0.33%,P<0.01),葛根总黄酮高剂量组斑块面积与管腔面积比值显著低于葛根总黄酮低剂量组(P<0.01)。结论葛根总黄酮一定程度抑制载脂蛋白E基因缺陷小鼠动脉粥样硬化斑块进展,该作用可能与降低小鼠血脂作用无明显关系。

The Effect of Total Flavone of Radix Puerariae on Atherosclerotic Plaque Formation of A polipoprotein E Gene Deficient Mice

Aim To investigate the effect of total flavone of radix puerariae on atherosclerotic plaque formation of aortic sinus in apolipoprotein E gene deficient(ApoE~(-/-)) mice. Methods 32 ApoE~(-/-) mice were divided randomly into four groups: model group,total flavone of radix puerariae low-dosage group(15 mg/kg everyday),total flavone of radix puerariae high-dosage group(85 mg/kg everyday) and atorvastatin calcium positive control group(5 mg/kg everyday).There were 8 mice in each group.The treatment group was stomach perfusion with relevant drug,the model group was stomach perfusion with equal amount of normal saline,and both group were medicated for 12 weeks and were fed with general diet.The routine biochemical method was used to measure total cholesterol,triglyceride and high-density lipoprotein-cholesterol in serum;and the serial paraffin section and Hematoxylin and eosin(HE) staining of aortic root were used to observe the histomorphological change of aortic sinus and measure the size of aortic sinus plaque in ApoE~(-/-) mice. Results A total of 65 plaques were detected in four groups.Despite the typical middle/advanced pathological changes of aortic sinus(such as fibrous plaque and/or atheromatous plaque),four groups were different in histological observation.In model group,some plaques were bigger,merged into flake,aortic wall thickened diffusively,aortic cavity was narrower.In otal flavone of radix puerariae treatment group and atorvastatin calcium positive control group,the aortic sinus had a more limited plaque lesion and a slighter narrowing of aortic cavity. The ratio of plaque area to aortic cavity area was significantly smaller in total flavone of radix puerariae high-dosage group,total flavone of radix puerariae low-dosage group and atorvastatin calcium positive control group than that in model group,and was significantly smaller in total flavone of radix puerariae high-dosage group than that in total flavone of radix puerariae low-dosage group(P<0.01). Conclusions Total flavone of radix puerariae can inhibit to some extent the progress of atherosclerotic plaque in ApoE~(-/-) mice,which might not be significantly related to its effect of decreasing mice blood lipid.