HBV在慢性乙型肝炎患者肝细胞染色体上整合情况的临床分析

采用荧光原位杂交(FISH)技术检测慢性乙型肝炎患者肝细胞染色体,明确是否存在HBV的整合及整合情况。选择我院慢性乙型肝炎患者共16例,制备其肝细胞染色体标本,以地高辛标记的HBV探针进行原位杂交。16例患者中,有5例患者的肝细胞分裂期染色体上可观察到绿色荧光信号,占31.25%,HBV呈低频率整合(1.00%~1.50%),整合位点呈随机分布。通过研究发现,应用FISH技术可检测肝细胞染色体上整合型HBV。慢性乙型肝炎患者肝细胞染色体上存在整合型HBV,使HBV感染可能发生成为一种遗传性传染性疾病,并成为肝癌高发的重要危险因素。

Detection of HBV virus integration in hepatacellular chromosome of the patients with chronic hepatitis B using fluorescence in-situ hybridization

To detect the integration of HBV on hepatacellular chromosome of the patients with chronic hepatitis B.using fluorescence in-situ hybridization 16 patients with chronic hepatitis B in our department were selected to set up the hepatacellular chromosome specimens.Chromosome in-situ hybridization was done with Dig-labeled HBV probe.Green fluorescent signals were seen in 5 patients specimens(31.25%).The integration sites were present in low frequency(1.00%-1.50%) and randomly distributied.Using fluorescence in-situ hybridization technique could detect HBV integration on the hepatacellular chromosome.HBV integrating into hepatacellular chromosome of the patients with chronic hepatitis B not only can lead to hereditary diseases,but also is a high risk factor for liver cancer.