| A study of DNA bases sequence preference of aclarubicin B to bind DNA and inhibit DNA-dependent RNA synthesis in vitro |
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| 作者姓名： | 李良宏 夏培元 王玉芬 洪诤 王浴生 |
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| 作者单位： | 华西医科大学 |
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| 摘 要： | 采用荧光淬灭法和体外无细胞ＲＮＡ合成系统研究了阿柔比星Ｂ结合ＤＮＡ和抑制ＤＮＡ依赖性ＲＮＡ合成的ＤＮＡ碱基顺序选择性。结果表明阿柔比星Ｂ与小牛胸腺ＤＮＡ，ｐｏｌｙ［ｄ（Ａ－Ｔ）］和ｐｏｌｙ［ｄ（Ｇ－Ｃ）］有明显结合；结合ＲＮＡ的活性小；与天然ＤＮＡ的结合力较与变性ＤＮＡ的结合力大。Ｓｃａｔｃｈａｒ分析显示阿柔比星Ｂ结合３种ＤＮＡ的亲和性依次为ｐｏｌｙ［ｄ（Ａ－Ｔ）］＞ｐｏｌｙ［ｄ（Ｇ－Ｃ）］＞小牛胸腺ＤＮＡ。同样，用大肠杆菌ＲＮＡ多聚酶和大鼠肝细胞核游离ＲＮＡ多聚酶实验均显示阿柔比星Ｂ的抑制力依次为ｐｏｌｙ［ｄ（Ａ－Ｔ）］＞ｐｏｌｙ［ｄ（Ｇ－Ｃ）］＞ｐｏｌｙ［ｄ（Ｉ－Ｃ）］。上述结果证明抑制ＲＮＡ合成的碱基顺序选择性与其结合ＤＮＡ碱基顺序的选择性有关
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| 关 键 词： | 阿柔比星B poly［d（A-T）］ poly［d（G－C）］ 荧光淬灭 RNA合成 DNA结合 |
A study of DNA bases sequence preference of aclarubicin B to bind DNA and inhibit DNA dependent RNA synthesis in vitro |
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| Li Lianghong Xia Peiyuan,Wang Yufen,Hong Zheng and Wang Yusheng.A study of DNA bases sequence preference of aclarubicin B to bind DNA and inhibit DNA-dependent RNA synthesis in vitro[J].Chinese Journal of Antibiotics,1998(3). |
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| Authors: | Li Lianghong Xia Peiyuan Wang Yufen Hong Zheng and Wang Yusheng |
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| Abstract: | |
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| Keywords: | Aclarubicin B Poly Poly [d (G-C)] Fluorescence quen ching RNA synthesis DNA binding |
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