移动抑制因子与体外受精-胚胎移植妊娠结局的相关性研究

目的:探讨人卵泡液中是否存在移动抑制因子(MIF)及其卵泡液和血液中的浓度对IVF-ET 妊娠结局的影响。方法:选择2004．08-2005．08行IVF-ET助孕的不孕妇女共37例,按妊娠结局分 为妊娠和非妊娠两组。收集取卵时的血液及卵泡液,定量酶联免疫法(ELISA)分别测定血及卵泡液 中MIF的浓度。并检测取卵前血中LH、P、E2值。结果:两组在获卵数、受精率、卵裂率、移植 胚胎数和激素水平无显著差异。妊娠组血清及卵泡液中MIF浓度分别为1．79±0．98 ng／ml和 22．97±10．62 ng／ml;非妊娠组分别为1．91±1．71 ng／ml和13．44±8．5ng/ml。两组卵泡液中 MIF浓度比较有显著差异(P<0．05);血清中浓度差异不明显(P>0．05)。卵泡液MIF浓度与优质胚 胎率呈正相关(r=0．3277,P<0．05),优质胚胎率在两组间差异显著(P<0．02)。结论:卵泡液中MIF可 能与卵泡发育及卵细胞质量有关,进而影响IVF-ET的结局。

Relationship between Macrophage Migration Inhibitory Factor and Pregnancy Outcome of IVF-ET

Object: To investigate the existence of MIF in follicular fluids (FF) and the correlation between pregnancy outcome after IVF and the concentration of MIF in periovulatory FF and blood. Method: FF and blood samples were obtained at oocyte retrieval from 37 women undergoing IVF-ET, from August 2004 to August 2005. They were divided into two groups basing on their pregnancy outcomes. Cytokines were measured by ELISA technique. Blood estradiol, progesterone and luteinizing hormone concentrations before the day of ovum pick-up were measured too. Result: A higher amount of MIF was identified in the FF of pregnancy group (22.97 ± 10.62 ng/ml) in which the concentration had significant difference from those of non-pregnancy group, (13.44 ± 8.5 ng/ml) (P<0.05), but there was no difference of the concentration in serum samples between these two groups (1.79 ± 0.98 ng/ml vs 1.91 ± 1.71 ng/ml). Besides, a positive correlation was observed between the follicular concentration of MIF and the rate of I grade embryo (r=0.3277, P<0.05). Conclusion: M(?) and granulosa cells may be the major source of MIF in human FF. Perhaps MIF plays an important role in the follicular development and the quality of oocytes, and its levels in FF appear to be associated with the IVF outcome.