口服谷胱甘肽对氟所致大鼠脂质过氧化的影响

目的 探讨口服不同剂量的谷胱甘肽（GSH）对氟所致大鼠质过氧化的影响。方法 对事先饮用10天150mg/L氟化钠（NaF）的雄性Wistar大鼠分别给予60、300和600mg/kg GSH灌胃12周，同时设对照组（饮蒸馏 水）和单纯氟染毒组（150mg/LNaF ），测定血清（全血）、肝、肾、心、睾丸和脑组织中超氧化物歧化酶（SOD） 活性和丙二醛（MDA）含量。结果 单纯氟染毒可使大鼠血清、肝、肾、脑MDA含量显著增加，全血、肝、肾、心、睾丸SOD活性显著降低；口服GSH后，血清、肝、肾、脑MDA的生成减少，肝、肾、心、睾丸和SOD活性显著上升，同是300mg/kgGSH可显著促进尿氟的排出。结论 氟可使大鼠脂质过氧化作用增强，抗氧化能力降低，口服GSH可拮抗氟致脂过氧化作用，增加抗氧化能力。

Effects of Oral Intake of Glutathione on Lipid Peroxidation Induced by Fluoride in Rats

Objective To explore the effects of oral intake of glutathione (GSH) with different concentrations on lipid peroxidation in rats Methods After drinking 150 mg/L sodium fluoride (NaF) solution for 10 days,the male Wistar rats were perfused perorally with glutathione at the doses of 60,300 and 600 mg/kg for 12 weeks respectively Meanwhile,the control group drinking distilled water and the exposure group drinking 150 mg/L NaF solution were established among male Wistar rats The activities of superoxide dismutase and the levels of malondialdehyde (MDA) in serum,whole blood,liver,kidney,heart,testis and brain tissue of rats were determined Results Simple exposure to fluoride could result in the significant increases of the levels of MDA in serum,liver,kidney and brain tissue of the rats,as well as the significant decreases of the activities of SOD in whole blood,liver,kidney,heart and testis tissue After the oral perfusion of GSH,the decreases of the levels of MDA were observed in serum,liver,kidney and brain,as well as the significant increases of the activities of SOD in liver,kidney,heart and testis The significant increase of the excretion of fluoride in urine was observed in rats perfused perorally with 300 mg/kg GSH Conclusion Fluoride could promote the lipid peroxidation and deteriorate the anti oxidative capacity,which could be antagonized by oral perfusion of GSH