细胞周期调控因子在中耳胆脂瘤上皮中的表达

目的根据细胞周期调控基因表达情况探讨中耳胆脂瘤上皮细胞增殖的分子机制。方法采用免疫组织化学方法检测胆脂瘤上皮细胞和胆脂瘤患者外耳道上皮细胞中周期蛋白依赖性激酶4(cyclindependentkinase4,CDK4)及其抑制因子p15、p16的表达,并结合炎症及骨质破坏程度作统计学分析。结果CDK4、p16在胞核、胞浆以棕黄色或棕褐色颗粒表达,并以胞核为主,而p15仅以胞核棕黄色或棕褐色颗粒表达。与皮肤相比,CDK4、p15、p16在胆脂瘤上皮细胞的表达显著增强,上皮下重度炎症可增强CDK4的表达;不同的骨质破坏程度上述指标的表达无显著性差异。结论胆脂瘤上皮细胞增殖活性增强,同时抑制细胞增殖的机制也增强;局部炎症可增强胆脂瘤上皮细胞的增殖活性。

Expression of cyclin dependent kinase4, p15, p16 in middle ear cholesteatoma epithelium

OBJECTIVE To study the cells prolif eration and its molecular regulating mechanisms of cho lesteatoma epithelium from the aspect of cell cycle control. METHODS The expressions of cyclin dependent kinase 4(CDK4),p15, and p16 were investigated by immunohistochemical S-P method and computer image analysis in 30 specimens of the cholesteatoma epithelium and 19 specimens of external ear canal epithelium from the patients with middle ear cholesteatoma. Statistical analyses were performed to study the correlation of the degree of subepidermal inflammatory cell infiltration and degree of bone destruction. RESULTS The staining of CDK4 and p16 were located in the nuclei and cytoplasm of cells, mainly in the nuclei, but the staining of p15 was expressed only in the nuclei of cells. The expressions of CDK4, p15, and p16 in cholesteatoma epithelium were significantly increased compared with that in external ear canal epithelium. The expression of CDK4 tended to be strong in the subepidermal inflammatory epithelium. The expressions of CDK4, p15, and p16 were not significantly different among the different degree of bone destruction. CONCLUSION The cholesteatoma epithelium have a unevenly hyperproliferation ability, and also show a mechanism of increased inhibiting proliferation ability. The inflammatory reaction increased the proliferation ability of cholesteatoma epithelium.