电化学发光免疫分析法和酶联免疫吸附法测HBsAb效果评价

用电化学发光免疫分析法(ECLIA)和ELISA分别测定不同浓度乙肝表面抗体(HBsAb),并对结果进行分析比较.结果表明: ECLIA法测HBsAb的批内CV: 高值为0.95%, 中值为1.13%, 低值为2.63%, 批间CV: 高值为4.03%, 中值为4.93%, 低值为7.34%, 灵敏度为4IU/L; ELISA法测HBsAb的批内CV: 高值为5.76%, 中值为12.8%,低值为15.9%,批间CV: 高值为10.1%, 中值为19.6%, 低值为25.2%, 灵敏度为19IU/L.5种用ELISA法筛选的165例标本, 除3例ECLIA法HBsAb阳性, ELISA法HBsAb阴性, 不符合外, 其他162例两种方法结果一致.总之,用ECLIA测定HBsAb的各技术参数优于ELISA法.

Evaluation of Electrochemiluminescene Immunoassay and Enzyme-linked Immunosorbent Assay for Serum HBsAb Detection

Electrochemiluminescene immunoassay(ECLIA) and enzyme-linked immunosorbent(ELISA) were used to detect the different concentrations of serum HBsAb, in order to compare the results of ECLIA and ELISA.The result showed that intra-assay coefficient of variation of ECLIA was about 0.95% for high value, 1.13% for mean values and 2.63% for low value, while that of ELISA was about 5.76%, 12.8% and 15.9%, respectively. The interassay coefficient of ECLIA was about 4.03% for high values,4.93% for mean values and 7.34% for low values, while that of ELISA was about 10.1%, 19.6% and 25.2%, respectively.The analytical sensitivity of ECLIA was about 4.0IU/L, while that of ELISA is about 19.0IU/L.Only in 3 samples, the results measured by ECLIA and ELISA were different(ECLIA: positive;ELISA:negative)among 165 samples.It is concluded that the method of ECLIA is more efficient than ELISA for detection of HBsAb in serum.