复合法建立大鼠溃疡性结肠炎模型

为建立一种类似人类溃疡性结肠炎（UC）特点的动物模型，将60只封闭群健康成年Wistar大鼠随机分为3组造模，每组20只，A组应用2，4－二硝基氯苯（DNCB）和乙酸复合法，B组单纯应用DNCB，C组单纯应用乙酸，对3组进行对照研究。结果：病程：A组大于16周，B组2周，C组8周；免疫学反应：造模前及造模完成后1周CD4＋CD29＋细胞A组为5．01±2．01％，11．17±2．18％，P＜0．01；B组为4．96±1．87％，10．98±2．87％，P＜0．01；C组为4．93±1.96％，5．06±2．03％，P＞0．05；病理学改变：3种方法均可产生粘膜溃疡、炎性细胞浸润、隐窝脓肿等UC组织学特征；症状：3种方法均可造成大鼠腹泻、粘法血便等UC症状。结论：复合法大鼠UC模型既克服了DNCB病程短、自愈性强的缺点，也克服了乙酸法不具有免疫反应模型特征的缺点，又同时保留了二者症状、组织学改变与人类UC相似的优点，是一种较理想的新的UC模型。

EXPERIMENTAL ULCERATIVE COLITIS PRODUCED BY COMBINED METHODS IN RATS

Objective To establish a successful chronic ulcerative colitis (UC) animal model similiar as human.Methods:Sixty Wistar rats rats were divided into 3 group eg. A [combined 2,4-Dinitrochloro benzene (DNCB) and aceticacid(AA)],B(DNCB only),C(AA only). Results 1.The course of models were 16 weeks in group A,2. weeks in group B and 8 weeks in group C;2. Immunoreactivity: before and 1 week of the model was performed , the CD4 CD29cell were 5.01% 2.01%, 11. 17% 2. 18% in group A (P <0.01),4.96% 1.87%, 10.98% 2. 87% ingroup B (P< 0.01),4.93% 1.96%,5 .06 2.03% in group C(P >0.05);3. Histology: mucosal congestion,netrophil infiltration,ulcer and abscess were observed in all three groups,Conclution.The UC model produced by combined DNCB and AA is successful and ressembles to human UC immunology in histologically.