胰岛素样生长因子受体靶向性基因导人系统的体外和体内导人瘤谱

目的 通过研究E5基因导入系统的体外、体内基因导入瘤谱,建成一个平台技术：一个基因导入系统可用于多种肿瘤的基因治疗。方法 应用E5基因导入系统在体外、体内转移报告基因到IGF I R阳性的多种肿瘤细胞与裸小鼠皮下肿瘤。结果 E5基因导入系统在体外转移报告基因到富于IGF I R的5种肿瘤细胞,在体内将报告基因转移到IGF I R阳性裸小鼠皮下肿瘤,其导入率与IGF I R表达水平呈正相关。而体外、体内均不能将报告基因导入到IGF I R阴性的肿瘤细胞和裸小鼠皮下肿瘤。结论 E5基因导入系统具有相对的靶向性与广谱性,在临床基因治疗研究和应用中具有潜在的价值。

In vitro and in vivo experiment of transduction of reporter gene into tumors by E5 gene delivery system

Objective To investigate the spectrum of tumors into which the E5 gene delivery system can transduce reporter gene so as to establish a platform technology that one gene delivery system can be used to treat multiple types of tumors. Methods The E5 delecvery system was used to transduce reporter genes into various types of IGF I R positive tumor cell lines in vitro and into different IGF I R positive tumors transplanted subcutaneously in nude mice in vivo. The efficiency of trasduction was examined. Results The E5 delivery system transferred reporter genes into five types of tumor cells that over express IGFIR in vitro and transferred reporter genes into different tumors transplanted subcutaneously in nude mice that over express IGFIR in vivo too. The transduction rate was positively correlated with the expression rate of IGFIR. However, the E5 delivery system failed to transfer reporter genes into tumor cedll lines in vitro and the tumors transplanted subcutaneously in nude mice in vivo, both of which were without IGF I R expression. Conclusion E5 delivery system has rather remarkable target ability and extensive usefulness. It has the potentiality in gene therapy of cancer.