| 薯蓣素抑制口腔鳞状细胞癌的机制研究 |
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| 引用本文： | 李明,赵卿,高峰.薯蓣素抑制口腔鳞状细胞癌的机制研究[J].中国现代医学杂志,2018,28(36):12-17. |
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| 作者姓名： | 李明 赵卿 高峰 |
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| 作者单位： | (1. 湖南省长沙市口腔医院, 湖南 长沙 410004 ；2. 中南大学湘雅三医院 超声科,
湖南 长沙 410013) |
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| 基金项目： | 长沙市科技计划项目（No ：kq1701018）；湖南省自然科学基金（No ：2018JJ2604） |
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| 摘 要： | 目的 研究薯蓣素对人口腔鳞状细胞癌的抑制作用。方法 薯蓣素处理TSCCa 和TCa8113 细胞，MTS
和软琼脂集落实验检测薯蓣素对口腔鳞状细胞癌增殖的影响。蛋白质免疫印迹检测薯蓣素对口腔鳞状细胞癌
糖酵解调控蛋白及凋亡调控分子表达的影响。检测过表达己糖激酶HK2 对薯蓣素诱导细胞凋亡的作用。结果
薯蓣素抑制TSCCa 和TCa8113 细胞的增殖具有剂量和时间依赖性（P <0.05）。薯蓣素抑制口腔鳞状细胞癌
糖酵解（P <0.05），并下调HK2 和葡萄糖转运蛋白Glut1 的表达。薯蓣素能诱导口腔鳞状细胞癌Caspase-3 和
PARP 剪切体表达上调，过表达HK2 抑制薯蓣素诱导的细胞凋亡。结论 薯蓣素对口腔鳞状细胞癌的抑制作用
与其下调HK2 和对糖酵解的抑制有关。
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| 关 键 词： | 口腔鳞状细胞癌 薯蓣素 己糖激酶 糖酵解 细胞凋亡 |
| 收稿时间： | 2018/4/17 0:00:00 |
Inhibitory effect of Dioscin on oral squamous cell carcinoma |
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| Ming Li,Qing Zhao,Feng Gao.Inhibitory effect of Dioscin on oral squamous cell carcinoma[J].China Journal of Modern Medicine,2018,28(36):12-17. |
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| Authors: | Ming Li Qing Zhao Feng Gao |
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| Institution: | (1. Changsha Stomatological Hospital, Changsha, Hunan 410004, China; 2. Department of
Ultrasonography, the Third Xiangya Hospital of Central South University,
Changsha, Hunan 410013, China)(1. Changsha Stomatological Hospital, Changsha, Hunan 410004, China; 2. Department of
Ultrasonography, the Third Xiangya Hospital of Central South University,
Changsha, Hunan 410013, China) |
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| Abstract: | Objective To investigate the inhibitory effect of Doscin on human oral squamous cell carcinoma
(OSCC). Methods TSCCa and TCa8113 oral squamous carcinoma cell line were treated with various concentration
of Dioscin. Cell proliferation was tested via MTS and soft agar colony formation assay. Cell glycolysis was analyzed.
Glycolysis and apoptosis related proteins were measured via immunoblotting assay. Western blot was performed to
determine the effect of hexokinase-2 (HK2) overexpression on Dioscin-induced cellular apoptosis. Results Dioscin
decreased the cell growth of TSCCa and TCa8113 cells in a dose- and time- dependent manner (P < 0.05). Dioscin
significantly suppressed glycolysis in TSCCa and TCa8113 cells and decreased the protein levels of Glut1 and HK2
(P < 0.05). Additionally, Dioscin elevated the expression of cleaved-caspase-3 and cleaved-PARP. Overexpression of
HK2 inhibited Dioscin-induced cell apoptosis. Conclusions Our results suggests that Dioscin inhibits OSCC growth
through inhibition of HK2 and glycolysis. |
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| Keywords: | oral squamous cell carcinoma Dioscin hexokinase-2 glycolysis apoptosis |
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