林下山参方酒精渗漉液对大鼠交配及勃起影响的研究

目的 探讨林下山参方酒精渗漉液（MCGPAP）对正常大鼠交配功能及肾阳虚大鼠勃起功能的影响。方法 以西地那非及丙酸睾酮作为阳性对照,雄性SD大鼠ip氢化可的松注射液复制肾阳虚模型,MCGPAP高、中、低剂量（2.25、1.125、0.562 5 mL/kg,换算为生药剂量为46.44、23.22、11.61 g/kg）连续给药14 d,检测MCGPAP对正常大鼠体质量、交配能力、精子数、性腺指数的影响;检测MCGPAP单次及连续给药14 d对肾阳虚大鼠体质量、勃起功能、性腺指数的影响。结果 MCGPAP对正常大鼠体质量、性腺指数无明显影响;与空白对照组比较,MCGPAP高、中、低剂量能增加正常大鼠30 min内插入次数及30 min内射精次数（P<0.05）;中剂量还能缩短插入潜伏期,高剂量能增加正常大鼠精子数,与空白对照、溶媒对照组比较均差异显著（P<0.05）。对肾阳虚大鼠,MCGPAP各剂量单次给药对其勃起功能、体质量无明显影响;MCGPAP给药14 d对大鼠体质量无明显影响;与模型组比较,中剂量能提高其勃起百分率、勃起次数（P<0.05）;MCGPAP低剂量组肾脏指数较模型组及溶媒对照组明显增加（P<0.05）,前列腺+精囊腺指数较溶媒对照组明显增加（P<0.05）。结论 林下山参方具有增强正常大鼠交配功能及肾阳虚大鼠勃起功能的作用。

Influence of mountain cultivated ginseng prescription alcohol percolate on rat mating and erectile

Objective To explore the effect of mountain cultivated ginseng prescription alcohol percolate (MCGPAP) on the mating function of normal rats and the erectile function of rats with kidney-yang deficiency. Methods Sildenafil and testosterone propionate were used as positive control. The kidney yang deficiency model was copied by ip injecting with hydrocortisone injection in male SD rats. MCGPAP was administered continuously for 14 days at high, medium and low doses (2.25, 1.125, 0.562 5 mL/kg, converted into raw dosage of 46.44, 23.22, 11.61 g/kg). The effects of MCGPAP on body mass, mating ability, sperm number and gonadal index of normal rats were examined. The effects of single and continuous administration of MCGPAP on body mass, erectile function and gonadal index of rats with kidney-yang deficiency were examined. Results MCGPAP had no significant effect on body mass and gonad index of normal rats; compared with blank control group, high, medium and low doses of MCGPAP could increase insertion times and ejaculation times within 30 minutes (P<0.05); middle doses could also shorten insertion latency, high doses could increase sperm numbers of normal rats, which were significantly different from blank control group and solvent control group (P<0.05). For kidney yang deficiency rats, single dose of MCGPAP had no significant effect on erectile function and body mass; 14 days of MCGPAP administration had no significant effect on body mass of rats; compared with model group, middle dose of MCGPAP could increase erectile percentage and erectile times (P<0.05); kidney index of low dose group was significantly higher than model group and solvent control group (P<0.05); prostate + seminal vesicle gland index was significantly higher than that of model group and solvent control group (P<0.05). Compared with the solvent control group, the number increased significantly (P<0.05). Conclusion s It shows that mountain cultivated ginseng prescription has the function of enhancing mating function of normal rats and erectile function of kidney yang deficiency rats.