糖尿病大鼠肾脏胱硫醚-β-合成酶和胱硫醚-γ-裂解酶mRNA表达初步研究

目的研究糖尿病大鼠肾脏内源性硫化氢(H2S)的两种合成酶胱硫醚-β-合成酶(cystathionine-β-synthase CBS)和胱硫醚-γ-裂解酶(cystathionine-γ-lyase CSE)mRNA表达.方法采用腹腔注射链脲佐菌素诱发糖尿病模型,大鼠随机分为两组:正常对照组(C组)和糖尿病组(D组).建模后第8周,测定24 h尿蛋白、血糖及肌酐清除率1],应用RT-PCR技术检测大鼠肾皮质CBS、CSE mRNA表达.结果糖尿病组大鼠尿蛋白、血糖及肌酐清除率均较正常对照组明显升高(P＜0.05),但肾皮质CBS、CSE mRNA表达较正常对照组无显著性差异(P＞0.05).结论CBS、CSE/H2S系统不能通过两种合成酶mRNA水平的改变而参与糖尿病肾病中血流动力学紊乱发生,其作用通路中的其他环节改变是否参与糖尿病肾病的发生发展尚需进一步证实.

Expression of cystathionine-β-synthase and cystathionine-γ-lyase in kidney of diabetic rat

Objective:To investigate the expression of enzyme cystathionine-β-synthase(CBS) and cys tathionine-γ-lyase(CSE) at mRNA level generated by hydrogen sulfide (H2S) in renal cortex of diabetic rats.Methods: The male SD rats were randomly divided into two groups: control group and diabetic group. After eight weeks, when the model was established, the excretion of urine protein, the level of blood glucose and the clearance of creatinine were measured. The expression of CBS and CSE at mRNA level in renal cortex w_ere detected by RT-PCR. Results: Urinary protein excretion, blood glucose and creatinine clearance increased in diabetic rats. But the expression of CBS and CSE at mRNA level had no significant differences in diabetic rats compared with those in control group. Conclusions: CBS, CSE/H2S couldn't play a role in the hemodynamic disorder of diabetic nephropathy by the change of two synthase at mRNA level. It is needed to demonstrate whether the change of other levels of the CBS, CSE/H2S pathway can take part in the diabetic nephropathy pathogenesis.