酶联免疫吸附试验检测血清结核抗体及临床意义

目的:探讨血清抗结核分支杆菌抗体检测在肺结核诊断中的临床意义,评价临床诊断试验的相关指标。方法:采用高纯度结核分支杆菌纯蛋白衍生物作包被抗原,应用ELISA检测临床诊断肺结核患者116例(其中分菌阳组、菌阴组、治疗后复检组)和112例非结核病患者,健康体检者的血清结核抗体水平;计算诊断试验的相关指标。结果:该检测方法临床诊断肺结核的敏感性为61.20%特异性为95.53%,准确性为78.07%,诊断指数为156.73%,阳性预期值为93.42%,阴性预期值为70.39%;各实验组与实验对照组间率的比较,均P

Measurement Of serum anti-mycobacterium tuberculosis antibody by ELISA and the clinical value

Objective To evaluate clinical value of measuring serum anti-mycobacterium tuberculosis antibody in detecting of pulmonary tuberculosis and the relevant indices of diagnostic experiment. Methods PPD from Mycobacterium tuberculosis taken as coating antigen,the antibody Of 116 patients with pulmonary tuberculosis ( thev were devided into positive bacteria group negative bacteria group and,remeasurement one afer treating)and 112 cases without pulmonary tuberculosis were measured by ELISA,to calculate the relevant indices. Results The sensitiviy was 61.20%,the specificity was95.53%,the accuracy was 78.07%, clinical indices was 156.73%,positive predictive value was 93.42%,and negative predictive value was 70.39%. The result of comparison of the rate of experiment group with that of control group was P<0.005?Conclusions The measurement of serum tuberculosis antibody by ELISA was proved to be better in.sensitivity and spectticity,can be used as auxiliary clinic and has high practical value in the curative effect of pulmonary tuberculosis.