超抗原SEA对Molt-4细胞线粒体膜电位的影响

目的:超抗原葡萄球菌肠毒素A(SEA)诱导Molt-4细胞活化与线粒体膜电位变化的关系。方法:应用CCK-8法体外检测不同浓度和时间SEA刺激传代T细胞株Molt-4细胞增殖活性,应用JC-1标记细胞线粒体,利用流式细胞术测定比较不同时间点SEA刺激对Molt-4细胞线粒体膜电位的影响。结果:经CCK-8检测,SEA的浓度在1 mg/L时Molt-4细胞的增殖最显著,以该浓度的SEA作用于Molt-4细胞180、60、30、10 min,10 min时间点膜电位升高明显高于其他时间点。SEA刺激10、30 min后线粒体膜电位升高低于有丝分裂原PHA刺激的结果。结论:SEA可诱导Molt-4细胞线粒体膜电位升高,且升高作用主要发生在细胞增殖的早期阶段。其升高作用略低于有丝分裂原PHA。

Effect of superantigen SEA on mitochondrial membrane potential of Molt-4 cell

AIM: To explore the effect of superantigen staphylococcal enterotoxin A(SEA) on mitochondrial membrane potential of Molt-4 cell.METHODS: Cell counting kit-8(CCK-8) was used to detect the proliferation of T cell in different concentration and time,We employed JC-1 to estimate mitochondrialmembrane potential(△ψm) of Molt-4 cell induced by SEA using flow cytometry(FCM).RESULTS: The proliferative activity of T cell treated with SEA(1 mg/L) was changed obviously compared with control.The mitochondrial membrane potential of Molt-4 cell with SEA(1 mg/L) was highest at 10 min by FCM after stimulation of 180,60,30 and 10 min.Mitochondrial membrane potential of Molt-4 cell treated with SEA after 10 and 30 min were lower than that with PHA which also have rising effect.CONCLUSION: Superantigen can enhance the mitochondrial membrane potential of Molt-4 cell in the early stage of proliferation.But the effect was weaker than that with mitogen PHA.