The behaviour of reduced proteins from normal and cataractous lenses in highly dissociating media: cross-linked protein in cataractous lenses |
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| Authors: | R H Buckingham |
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| Affiliation: | 1. College of Chemistry and Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, Xiangtan University, Xiangtan 411105, Hunan Province, China;2. College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing 100049, China;1. Nutrition and Food Science, Department of Physical Sport Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia;2. Department of Food Science and Nutrition, College of Food and Agricultural Science, King Saud University, Riyadh, Saudi Arabia;3. Department of Basic Medical Sciences, College of Medicine, King Saud bin Abdulaziz University for Health Sciences (KSAU-HS), Riyadh, Saudi Arabia;4. King Abdullah International Research Center (KAIMRC), King Saud Bin Abdulaziz University for Health Sciences (KSAU-HS), Riyadh, Saudi Arabia;1. School of Biological Sciences, The University of Hong Kong, Hong Kong 999077, China;2. State Key Laboratory of Organ Failure Research, Department of Biostatistics, School of Public Health, Southern Medical University, Guangzhou 510515, China;3. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China |
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| Abstract: | Reduced proteins from normal and cataractous lenses have been examined in 6 m-guanidinium chloride and in sodium dodecyl sulphate as complexes with the detergent. Under these conditions normal lens proteins dissociate completely to polypeptide chains of low molecular weight.Cataractous lenses with golden-yellow nuclear cores (group III lenses) yeild 15–20% of their protein in a form which is resistant to such dissociation. It appears that these aggregates, with a molecular weight above 5 × 104, are maintained by covalent bonds which are not disulphide bridges. These observations are supported by electrophoresis of reduced lens proteins as complexes with sodium dodecyl sulphate in polyacrylamide gel. The protein of high molecular weight from cataractous lenses is derived exclusively from the lens nucleus. The coloration of group III lenses is associated mostly with the cross-linked protein. |
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