塞替派诱发人支气管上皮恶性转化成瘤细胞的染色体畸变

目的　旨在了解转化细胞在成瘤过程中的细胞遗传学改变。方法　运用染色体G显带技术研究永生化人支气管上皮细胞 (BEAS 2B)恶性转化后的裸小鼠接种成瘤细胞 (BEAS TT)的染色体畸变。结果　瘤细胞在传代早期基本以近二倍体细胞为主 ,随着细胞代龄的增加 ,各肿瘤细胞系的细胞染色体数目变化趋势不同 ,其中BEAS TTa逐渐形成以多倍体细胞为主的细胞群 ,而BEAS TTb ,BEAS TTc则以近二倍体细胞为主份额细胞。核型分析表明 3个瘤细胞系的核型与BEAS TE不同 ,在其基础上有新的染色体 (14号染色体 )丢失和标记染色体 (M4 )的增加。结论　细胞染色体数目不稳定 ,14号染色体的丢失和M4染色体的增加 ,可能与BEAS TE的裸小鼠成瘤性有关

Chromosome aberration of tumorigenic human bronchial epithelial cells induced by thiotepa

AIM To analyze cytogenetical changes in transformed cells in tumorigenesis. METHODS G-banding technique was used to analyze the karyotype of BEAS-TTs. BEAS- TTa, BEAS-TTb and BEAS-TTc are tumor cells derived from subcutaneously implanted malignantly transformed immortalized non-tumorigenic human bronchial epithelial cells by thiotepa (BEAS-TE) in nude mice. RESULTS The modal chromosomal number of three tumor cells was near diploid at passage 7, 6, 6 for tumor cell BEAS-TTa, BEAS-TTb and BEAS-TTc, respectively. Proceeding with subcultures， there was a shift for BEAS-TTa from near diploid toward poly-ploid at passage 27，the proportion of poly- ploid cells was up to 96%, while the BEAS-TTb and BEAS-TTc were maintained near diploid. On the chromosomal basis of BEAS-2B and BEAS-TE，BEAS-TT contin ued to loss the chromosome 14 and showed a new abnormal chromosome, the marker 4. CONCLUSION The progressive chromosomal changes that occurred during tumorigenesis process in nude mice was significantly related to BEAS-TE acquisition of strong tumorigenicity in nude mice.