抗氧化剂在保护造血干细胞生物学功能方面的作用简

在外周血造血干细胞移植中,骨髓造血干细胞（bone marrow hematopoietic stem cells,BMHSC）的动员及其在外周血液高氧环境中的循环都增加了活性氧（reactive oxygen species,ROS）的产生,这对BMHSC的生物学功能起到了抑制性的作用.本研究在BMHSC的培养中,应用维生素C的衍生物——抗坏血酸磷酸酯盐（ascorbic acid 2-phosphate,AA2P）来抑制ROS产生,并模仿其在外周血造血干细胞移植中的氧环境,通过体外增殖实验、流式分选技术、活性氧测定、CD34＋造血干细胞移植等实验来评价上述培养方法对BMHSC生物学功能的保护作用.结果表明：体外培养的BMHSC的ROS水平明显高于新鲜分离的BMHSC;同时,扩增的AC133＋ CD34＋早期红系细胞集落形成单位（BFU-E）、粒-单核细胞集落形成单位（CFU-GM）、粒-单核细胞混合集落形成单位（CFU-GEMM）的数量、细胞迁移率、人重症联合免疫缺陷鼠（SCID）移植重建细胞的数量都要明显高于在不含AA2P的环境中培养的造血干细胞.结论：抗氧化剂的干预是在外周血造血干细胞移植过程中保护HSC生物学功能的一种有效手段,并能够提高外周血造血干细胞移植的治疗效果.

Role of Antioxidant in Protecting the Biological Function of Hematopoietic Stem Cells

In periheral blood hematopoietic stem cell transplantation（PBHSCT）,the mobilization and circulating of bone marrow hematopoietic stem cells in blood with higher oxygen concentration all increase reactive oxygen species （ROS） production,which has negative effect on the biological function of BMHSC.In order to investigate the protective effect of antioxidant on hematopoietic stem cells （HSC）,the ascorbic acid 2-phosphate （AA2P）,an ascorbic acid derivative of vitamin C,was added in HSC culturing by initating oxygen conditions which BMHSC experienced in peripheral blood stem cell transplantation.The protective effect of above-memioned culfure methods on the biologied functions of BMHSC was evaluated by vitro amplification assay,committed division assay,reactive oxygen species （ROS） measurement,CD34＋ HSC engraftment.The results showed that the ROS level in HSC from in vitro cultures was much higher than that freshly separated BMHSC,and the amplified AC133 ＋ CD34＋ HSC,BFU-E,CFU-GM,CFU-GEMM colonies,migration rate and severe combined immunodeficiency （SCID）-repopulating cells （SRC） were all much more than HSC cultured without AA2P.It is concluded that antioxidant intervention may be an effective methods for protecting the biological function of PBHSC and improving the therapeutic effect of PBHSCT.