骨实质间充质干细胞在小鼠肺与骨髓中的分布简

本研究探讨慢病毒质粒pGC FU-RFP-LV标记的小鼠骨实质来源的间充质干细胞在小鼠肺组织和骨髓中的分布.将感染效率为78％的慢病毒感染小鼠MSC,尔后将感染后的MSC以1×106个/只的浓度经小鼠尾静脉植入到BALB/c小鼠体内.随机将小鼠按照1、2、5、7d4个时间点分为4组.在各时间点取小鼠肺组织并制成冰冻切片及骨髓涂片,观察其分布情况.结果表明：慢病毒感染后的MSC经免疫表型分析和诱导分化检测均符合MSC的表型及生物学特征.以最适病毒滴度MOI=50感染小鼠MSC后,细胞生长状态羌明显改变.通过荧光显微镜观察小鼠第1、2、5、7天MSC在骨髓中分布为（0.50±0.20）,（0.67±0.23）,（0.53±0.14）,（0.33±0.16）;在肺组织中分布为（0.55±0.15）,（0.47±0.13）,（0.29±0.13）,（0.26±0.08）.结论：MSC在第1天主要分布在肺组织内（P＜0.05）,而在第2天主要分布在骨髓中（P＜0.05）.小鼠骨实质MSC分布浓度与RFP基因表达及MSC在肺组织和骨髓中定植情况有关.

Distribution of Compact Bone Mesenchymal Stem Cells in Lung tissue and Bone Marrow of Mouse

This study was aimed to investigate the distribution of compact bone mesenchymal stem cells （MSC）marked with lentiviral plasrnid pGC FU-RFP-LV in lung tissue and bone marrow of mouse.The MSC were infected by lentivirus with infection efficiency 78％,the infected MSC were injected into BALB/c mice via tail veins in concentration of 1 x 106/mouse.The mice were randomly divided into 4 group according to 4 time points as 1,2,5 and 7 days.The lung tissue and bone marrow were taken and made of frozen sections and smears respectively in order to observed the distribution of MSC.The results indicated that the lentiviral infected MSC displayed phenotypes and biological characteristics which conformed to MSC by immunophenotyping analysis and induction differentiation detection.After the MSC were infected with optimal viral titer MOI =50,the ceil growth no significantly changed; the fluorescent microscopy revealed that the distributions of MSC in bone marrow on day 1,2,5 and 7 were 0.50 ± 0.20,0.67 ± 0.23,0.53 ± 0.14,0.33 ± 0.16; those in lung tissue were 0.55 ± 0.15,0.47 ± 0.1 3,0.29 ± 0.13,0.26 ± 0.08.It is concluded that the distribution of MSC in lung tissue reaches a peak on day 1,while distribution of MSC in bone marrow reaches a peak on day 2.The distribution of mouse MSC relates with RFP gene expression and implantation of MSC in lung tissue and bone marrow.