miR-21在弥漫大B细胞淋巴瘤肿瘤组织中的表达及临床意义

本研究旨在检测弥漫大B细胞淋巴瘤（DLBCL）患者肿瘤石蜡组织中miR-21的差异性表达与PTEN相关性及其临床意义。采用Real-time-PCR方法检测26例DLBCL患者及10名正常对照的miR-21表达，应用聚合物免疫组化检测系统检测PTEN蛋白水平。结果表明，26例DLBCL石蜡组织中miR-21的表达量为6．586（1．10，38．22），显著高于正常对照0．791（0．35，2．87）]（P〈0．05），在26例DLBCL中有6例PTEN蛋白（23％）阳性，而在20例（77％）中为阴性。DLBCL中miR-2l表达水平与PTEN蛋白水平呈负相关，其高表达与DLBCL血清LDH水平呈正相关、Ⅲ／Ⅳ期患者miR-21表达水平明显高于Ⅰ／Ⅱ期患者（P〈0．05），与临床亚型无关（P〉0．05）。结论：在DLBCL中miR-21表达升高可能提示肿瘤恶性程度高，预后不良，PTEN可能是miR-21的靶基因。

Expression and Clinical Significance of miR-21 in Diffuse Large B Cell Lymphoma

This study was aimed to investigate the expression of microRNA-21 and its correlation with PTEN in dif- fuse large B cell lymphoma（DLBCL） paraff＇m-embedded tissues, and evaluate its potential relevance with clinical char- acteristics. The expression levels of miR-21 in 26 primary DLBCL and 10 normal lymphnode tissue specimens were ex- amined by real-time polymerase chain reaction. The expression of PTEN was detected by immunohistochemical staining. The results indicated that the expression of miR-21 was significantly higher in tumor tissues 6.586 （1.10,38.22） ] than that in normal tissues 0.791 （0.35,2.87） ] （ P 〈 0.05 ）. Among 26 patients with DLBCL the expression of PTEN pro- tein was po- sitive in 6 patients（23% ）, and was negative in 20 patients（77% ）. In patients with DLBCL, the expression level of miR-21 was negatively correlated with the level of PTEN protein. The high expression of miR-21 was possitively correlated with the level of serum LDH. The expression level of miR-21 in patients with Ann Arbor Ⅲ - Ⅳstage was obviously higher than that of patients with Ann Arbor Ⅰ - Ⅱ stage, but did not correlate with the subtype of patients in clinic（ P 〉 0.05 ）. It is concluded that the expression of miR-21 is high in DLBCL and its overexpression may be related with poor prognosis of DLCBL. These findings suggest that PTEN is possibly one of the targets of miR-21 in DLBCL.