和厚朴酚联合吉西他滨协同抑制 Burkitt 淋巴瘤细胞增殖和促凋亡作用简

本研究旨在探讨和厚朴酚（Honokiol,HNK）与吉西他滨（Gemcitabine,GEM）联合应用对Burkitt淋巴瘤细胞株Raji细胞增殖和凋亡的影响.应用CCK-8法检测HNK和GEM单用及联合应用对Raji细胞增殖的抑制作用;采用流式细胞术检测两种药物诱导Raji细胞凋亡情况及细胞周期的变化;以Western blot法检测BCL-2抗凋亡蛋白水平的变化.结果表明,HNK与GEM联合后,细胞生长抑制明显高于HNK和GEM单药组（P＜0.01）,具有时间和浓度依赖性;细胞凋亡比例均较各单药组增多,差异具有统计学意义（P＜0.01）,两药联合后大部分细胞被阻滞在G0/G1期,S期细胞减少;细胞内抗凋亡蛋白BCL-2水平在联合组比在各单药组表达量下降,差异具有统计学意义（P＜0.01）.结论：HNK联合GEM具有协同抑制Burkitt淋巴瘤Raji细胞增殖并诱导其凋亡的作用,协同机制可能与两药联合后更多细胞发生G0/G1期阻滞,并抑制抗凋亡蛋白BCL-2的活化有关.

Honokiol combined with Gemcitabine Synergistically Inhibits the Proliferation of Human Burkitt Lymphoma Cells and Induces their Apoptosis

This study was aimed to investigate the effect of Honokiol（HNK） combined with Gemcitabine（GEM） on the proliferation and apoptosis of human Burkitt lymphoma Raji cells.Cell proliferation was detected by CCK-8 method to study the role of Honokiol and Gemcitabine in Raji cells.The cell apoptosis and cell cycle status were analyzed by flow cytometry.The level of apoptosis-related protein BCL-2 was measured with Western blot.The results showed that compared with cells treated with mentioned above drugs alone,the proliferative potential of cells in combination group was significantly inhibited（P ＜0.01） and the inhibition rate was related to the concentration and action time of HNK; and apoptosis rate markedly increased（P ＜ 0.01）,while most Raji cells were arrested at G0/G1 phase and decreased in S phase after treatment with combination of two drugs; the expression of BCL-2 protein decreased （P ＜ 0.01）.It is concluded that Honokiol combined Gemcitabine can synergistically inhibit the proliferation,induce cell apoptosis,and down-regulate the expression of BCL-2 in Raji cells.The possible mechanism of synergistic effect may be related with arrest of cell cycle at G0/G1 phase and downregulation of the expression of BCL-2.