miR-16 在 T 淋巴母细胞淋巴瘤 / 急性淋巴母细胞白血病中表达简

本研究探讨miR-16在T淋巴母细胞淋巴瘤/急性淋巴母细胞白血病（T-LBL/ALL）中的表达及其与预后的关系.应用免疫组织化学法对宜共市人民医院血液科有详细随访资料的38例T-LBL/ALL石蜡标本进行CD3、cCD3、CD10、CD20、CD34、CD43、CD99、TdT、PAX-5、BCL-2和Ki67免疫组织化学标记检测,采用real-time RT-PCR方法检测miR-16的表达水平,以15例淋巴结反应性增生作为对照.结果表明：38例T-LBL/ALL中TdT阳性率最高（94.7％）,CD34阳性最低（22.1％）,PAX-5及CD20为阴性.在39.5％病例中Ki67大于80％.与淋巴结反应性增生相比较,miR-16在T-LBL/ALL中表达上调,其表达量是淋巴结反性增生的4.87倍（P＜0.05）.T-LBL中miR-16高表达组总体生存率下降（P＜0.05）.BCL-2蛋白表达阳性组预后优于阴性组预后（P＜0.05）.miR-16表达与BCL-2蛋白存在相关性（r=0.51,P＜0.05）.结论：在T-LBL/ALL中miR-16的高表达组总体生存率明显高于低表达组,提示miR-16可能与预后有相关性,而BCL-2蛋白表达阳性组预后好于阴性组,可能也是一种影响预后的因素.

Expression of miR-16 in Patients with T Lymphoblastic lymphoma/Acute Lymphoblastic Leukemia

This study was purposed to investigate the expression of miR-16 in T lymphoblastic lymphoma/acute lymphoblastic leukemia （T-LBL/ALL） and its relation with target therapy and prognosis.The CD3、cCD3 、CD10、CD20 、CD34、CD43、CD99、TdT、PAX-5、BCL-2 and Ki67 in paraffin samplcs from 38 cases of T-LBL/ALL were detected by immunohistochemical labeling; the miR-16 expression level was detected by real-time RT-PCR.Fifteen cases of reactive hyperplasia of lymphonodes were selected as control.The results indicated that among 38 cases of T-LBL/ALL the positive rate of TdT was highest （94.7％）,the positive rate of CD34 was lowest （22.1％）,the PAX-5 and CD20 were found to be negative.The Ki67 expression level in 39.5％ cases exceeded 80％.As compared with reactive hyperplasia of lymphonode,the miR-16 expression in T-LBL/ALL was up-regulated,ant its expression level was 4.87-fold of reactive hyperplasia of lymphonode （P ＜ 0.05）.The overall survival rate in group of miR-16 high expression decreased （P ＜ 0.05）.The prognosis of T-LBL/ALL patients with BCL-2 positive expression was better than that of patients with BCL-2 negative expression （P ＜ 0.05）.The miR-1 6 expression correlated with BCL-2 protein （r =0.51,P ＜0.05）.It is concluded that the overall survival rate in miR-16 high expression group is higher than that in miR-16 low expression group,suggesting possible relation of miR-16 with prognosis.Moreover,the prognosis in BCL-2 positive expression group is better than that in negative expression group,which may be a factor influencing prognosis.