MMP-2和MMP-9在急性B淋巴细胞白血病中的表达及临床意义

本研究探讨急性B淋巴细胞白血病（B-ALL）患者骨髓细胞中基质金属蛋白酶-2和-9（MMP-2，MMP-9）的表达及临床意义。采用半定量RT-PCR方法检测初治、复发B-ALL患者和正常对照骨髓中单个核细胞MMP-2，MMP-9mRNA的表达。采用明胶酶谱检测B-ALL患者和正常对照骨髓单个核细胞孵育上清中MMP-2，MMP-9的酶活性。结果表明，初治、复发组B—ALL患者中MMP-2表达率明显高于正常对照组（P〈0．05）；MMP-9表达率明显低于正常对照组（P〈0．05）。髓外浸润组患者MMP-2和MMP-9表达率均明显高于无髓外浸润组（P〈0．05），而MMP-2／MMP-9（＋）组髓外浸润发生率明显高于MMP-2／MMP-9（-）组。高危组B—ALL患者MMP-9表达率明显高于标危组，而两组间MMP-2表达率无显著性差异（P〉0．05）。结论：B-ALL患者白血病细胞可以产生MMP-2，MMP-9，可能参与了B-ALL的髓外浸润，MMP-9表达可能提示预后不良。

Expression and Clinical Significance of MMP-2 and MMP-9 in B Acute Lymphoblastic Leukemia

This study was purposed to investigate the expression and clinical significance of MMP-2 and MMP-9 in patients with B-acute lymphoblastic leukemia （B-ALL）. The expression of MMP-2 and MMP-9 in bone marrow mononuclear cells of B-ALL patients and normal controls was detected by RT-PCR. The gelatinolytic activity was detected by zymography. The results showed that the expression of MMP-2 in de novo and relapsed B-ALL patients was markedly higher than that in normal controls （P 〈0.05）. The expression of MMP-9 in de novo and relapsed B-ALL patients was markedly lower than that in normal controls （ P 〈 0.05 ）. The expression of MMP-2 and MMP-9 in patients with extramedullary infiltration was significantly higher than that in patients without extramedullary infiltration. The incidence of extramedullary infiltration in patients with MMP-2/MMP-9 （ ＋ ） was markedly higher than that in patients with MMP-2/ MMP-9 （ - ）. The expression of MMP-9 was markedly higher in high-risk patients than that in standard-risk patients （ P 〈 0.05 ）, but the expression of MMP-2 had no significant difference between the high-risk and standard-risk patients （P 〉 0.05 ）. It is concluded that MMP-2 and MMP-9 may be secreted by B lymphoblasts and may involve in the extramedullary infiltration. MMP-9 may correlate with ooor prognosis.