TRIzol法与磁珠法用于丙型肝炎病毒RNA定量检测的比较

目的 比较TRIzol法与磁珠法对丙型肝炎病毒(HCV) RNA定量检测结果的影响.方法 收集117例丙型肝炎病毒感染阳性患者的血清及其基因分型信息.分别采用TRIzol法与磁珠法提取患者血清样本的HCVRNA,qPCR检测提取HCV RNA的病毒载量,分析两种提取方法所得HCV RNA检测结果的差异.结果 qPCR结果显示TRIzol法和磁珠法具有良好的线性相关性:y=0.978x+0.063(R2 =0.973).Bland-Altman统计分析显示TRIzol法提取的HCV RNA病毒载量对数值的平均值略低于磁珠法,但差异无统计学意义(P＞0.05).基因型分析结果显示1a、1b、2a、3a和6a基因型中两种提取方法差异均无统计学意义(P＞0.05).结论 TRIzol法提取HCV RNA的定量检测结果和磁珠法相当,且成本低廉,可在国内广泛用于试剂盒的开发和HCV RNA临床检测.

Comparison of TRIzol method and magnetic beads method in quantitative detection of hepatitis C virus RNA

Objective To compare the effects of TRIzol and magnetic beads methods on quantitative detection of hepatitis C virus (HCV) RNA.Methods Serum samples and genotype information of 117 patients with positive HCV infection were collected.HCV RNA was extracted from serum samples by TRIzol method and magnetic bead method,respectively.And then the viral load of HCV RNA was detected by quantitative PCR to compare the difference between the two methods.Results qPCR results showed that a good linear correlation existed between TRIzol and moagnetic beads methods:y=0.978x+0.063 (R2 =0.973).Bland-Altman statistical analysis showed that the average logarithmic value of viral load of HCV RNA extracted by TRIzol method was slightly lower than that of magnetic beads method,without significant difference (P＞0.05).There were no significant difference among the genotypes 1a,1b,2a,3a or 6a between the two methods (P＞0.05).Conclusion TRIzol method is comparable to magnetic beads method in HCV RNA quantitative detection,with less sample volume and lower cost,indicating that it might be widely used for developing kit and HCV RNA clinical detection in China.