腰椎间盘组织中MMP-7的表达及意义

目的 检测突出间盘组及对照组腰椎间盘中基质金属蛋白酶-7(MMP-7)的表达情况，探讨MMP-7在腰椎间盘退变突出中的作用。方法 应用免疫组化链霉素亲生物素-过氧化物酶法(S-P)对实验组32例腰椎间盘突出标本及对照组11例非突出间盘标本分别进行抗MMP-7蛋白单克隆抗体染色。实验组按术中病理分型分为突出、脱出、游离型。显微图象分析系统采集灰度值，数据进行统计学分析。结果 实验组MMP-7蛋白表达高于对照组，实验组中游离型及脱出型的MMP-7蛋白表达均比突出型高，游离型和脱出型之间MMP-7蛋白表达无差异。结论 腰间盘组织中MMP-7蛋白表达情况与腰椎间盘退变突出有关，MMP-7蛋白表达增加可能是导致腰间盘退变突出的原因之-。

Expression and Significance of Metalloproteinase-7 in Lumbar Intervertebral Disc

Objective To detect the expression of the MMP-7 protein in lumbar intervertebral disc and discuss the role of the MMP-7 protein in the course of the intervertebral disc degeneration.Methods Immunohistochemistry S-P method was used in experiment group which contains thirty two prolapse intervertebral discs and control groups which contains eleven discs . MMP-7 monoclonal antibodies stain the section and Gray Scale Value were collected by using microimage analysis system .Results The expression of MMP-7 in the experiment group is obviously higher than in the control group . There are statistical significance between extrusion or sequestration type and protrusion type , but no statistical significance between extrusion and sequestration type in experiment group . Conclusions There are obvious correlation between the expression of MMP-7 protein and disc degeneration and prolapse in lumbar intervertebral disc.The increase of the expression of MMP-7 protein maybe cause the disc degeneration and prolapse .