血吸虫不同感染频次对宿主肝纤维化程度的影响

目的以等量血吸虫尾蚴分别进行1次性和分2次、3次、4次感染4组小鼠,建立小鼠血吸虫感染模型,观察不同感染频次对肝纤维化程度的影响。方法通过肝脾指数来判断肝损害程度;化学发光法检测血清中透明质酸（HA）;病理切片HE染色观察胶原沉积、纤维形成情况和形成率。结果 1次性感染组肝纤维化形成率及纤维化程度不高,肝损害较轻;2次以上感染组,18周后所有小鼠均有肝脏纤维化的形成,纤维化程度与感染频次呈正相关,肝损害程度随感染频次增加而加重。结论血吸虫反复感染可导致患者严重肝损害,患者一旦被血吸虫尾蚴感染应及时治疗,避免反复多次感染。

Impact of Schistosoma japonicum Infection Frequency on the Degree of Liver Fibrosis in Hosts

Objective To observe the infection frequency of Schistosoma japonicum on liver injury through establishing the mouse models of schistosomiasis. Methods The mice were infected with the same cercarie by 1,2,3 and 4 times,respectively.Liver index and spleen index were calculated and hyaluronic acid(HA) was detected by chemiluminescence to judge liver injury.Pathological sections and hematoxylin and eosin stain were made to observe collagen deposition and the rate of fibrosis formation in mice livers. Results The lower rate and degree of fibrosis formation were observed in 1 time infection group,but fibrosis formation was observed in all mice livers which were infected more than 2 times,and the degree of liver injury was positively correlated with the infection frequency. Conclusions Repeated infection of Schistosoma japonicum can induce severe liver injury.Persons infected with schistosome cercariae should be treated at once and also should avoid repeated infection.