丙烯酰胺对PC12细胞损伤及机制研究 |
| |
引用本文: | 李程,王穆,吴剑阁,何翠琳,李盼盼,杨慧仙,让蔚清,何爱桃. 丙烯酰胺对PC12细胞损伤及机制研究[J]. 实用预防医学, 2011, 18(10): 1826-1829 |
| |
作者姓名: | 李程 王穆 吴剑阁 何翠琳 李盼盼 杨慧仙 让蔚清 何爱桃 |
| |
作者单位: | 1. 南华大学公共卫生学院环境医学与放射卫生研究所,湖南衡阳,421001 2. 衡阳市疾病预防控制中心 3. 南华大学公共卫生学院2006级卫生检验专业 |
| |
基金项目: | 衡阳市社会发展科技支撑计划项目(2009ks31) |
| |
摘 要: | 目的探讨丙烯酰胺(AA)对PC12细胞的细胞毒性及可能机制。方法分别以0、62.5、125、250、500、1 000μmol/L AA为染毒剂量,染毒12 h后,采用MTT比色法检测丙烯酰胺对体外培养PC12细胞的增殖抑制作用,并用碱性单细胞凝胶电泳技术(single cell gel electrophoresis,SCGE)检测PC12细胞DNA的损伤作用、用MDA和T-AOC试剂盒检测细胞MDA和T-AOC改变。结果染毒12 h后,细胞生长受到明显的抑制,且染毒剂量越高,抑制作用越明显(P〈0.01);单细胞凝胶电泳实验表明,AA对体外培养PC12细胞的DNA有明显的损伤作用,细胞上清液中MDA含量随着染毒量的上升而升高,T-AOC含量随染毒量的上升而降低。结论 AA能够显著抑制PC12细胞活性,损伤细胞DNA,并诱导细胞氧化损伤。
|
关 键 词: | 丙烯酰胺 PC12细胞 MTT比色法 单细胞凝胶电泳技术 丙二醛 总抗氧化能力 |
Research on Injury Effect of Acrylamide on PC12 Cells and Its Mechanism |
| |
Affiliation: | LI Cheng,WANG Mu,WU Jian-ge,et al.(The Institute of Environmental Medicine and Radiology,School of Public Health,University of South China,Hengyang 421001,Hunan,China) |
| |
Abstract: | Objective To explore the cytotoxicity of acrylamide(AA) to PC12 cells and the possible mechanism. Methods Cells were exposed to 0,62.5,125,250,500,and 1,000 μmol/L AA,respectively,and after 12 hrs,the MTT method was used to detect the rate of cell proliferation and inhibition,alkaline single-celled gel electrophoresis technology was harnessed to obtain the result of DNA damage level of PC12 cells,and the MDA and T-AOC kit were applied to determine the content change of MDA and T-AOC in cell cultures. Results After 12 hrs AA administration,the growth of cells was significantly inhibited.Single-celled gel electrophoresis experiment showed that the DNA of PC12 cells exposed to AA was significantly damaged.MDA content in cell culture was elevated with AA dose rising,while T-AOC content was reduced with AA dose rising.And the aforementioned toxic effect was statistical significance(P<0.01). Conclusions AA can significantly inhibit cell activity,cause DNA damage,and induce cells oxidative damage in PC12 cells. |
| |
Keywords: | Acrylamide PC12 cells MTT SCGE MDA T-AOC |
本文献已被 CNKI 维普 万方数据 等数据库收录! |
|