原花青素对氨基脲染毒小鼠免疫细胞活性的影响

目的观察原花青素（PC）对氨基脲（SEM）染毒小鼠免疫细胞活性的影响。方法将50只成年KM小鼠,随机分为5组：溶剂对照组,SEM染毒组,SEM染毒＋低、中、高剂量PC保护组;溶剂对照组,灌胃去离子水;SEM染毒组,前两周灌胃56.25 mg/kg.bw SEM溶液,后4周灌胃去离子水;低、中、高剂量PC保护组,前2周均灌胃56.25 mg/kg.bw SEM溶液,后4周分别灌胃100、200、400 mg/kg.bw PC溶液。末次灌胃24 h后,摘眼球采血后处死小鼠,测定相关检测指标。结果 SEM染毒组小鼠NK细胞活性、腹腔巨噬细胞的吞噬能力、抗体形成细胞数、淋巴细胞转化能力（SI）均低于溶剂对照组（P〈0.05）;而中、高剂量PC保护组小鼠NK细胞活性、腹腔巨噬细胞的吞噬能力、AFC及SI均高于SEM染毒组,其差异均有统计学意义（P〈0.05）。结论适量的原花青素可减轻氨基脲致小鼠免疫细胞活性的损伤。

Effect of Procyanidine on Semicarbazide-induced Immune Injury in Mice

Objective To observe the effect of procyanidine(PC) against immune injure induced by semicarbazide(SEM) on mice. Methods Fifty adult KM mice were equally divided into 5 groups,including solvent control group,SEM exposure group,the exposure groups of SEM plus different PC doses.The mice of solvent control group were given distilled water once a day for 6 weeks,while those of SEM exposure group were gavaged with 56.25 mg/kg·bw SEM solution in the first two weeks,then fed with deionized water for four weeks.The mice in the exposure groups of SEM plus different PC doses were fed with 56.25 mg/kg·bw SEM solution in the first two weeks,then 100 mg/kg·bw,200 mg/kg·bw,400 mg/kg·bw PC solution ensued respectively.The immune relevant indictors were measured by relative tests after the last gavage. Results The activity of NK cell and macrophage,the number of antibody forming cells,lymphocyte proliferation ability in SEM exposure group were all lower than those in solvent control group(P<0.05),while those in moderate-and high-dose PC solution groups were all higher than those in SEM exposure group,and the differences were statistically significant(P<0.05). Conclusions Appropriate dose of PC can protect against the immune injury induced by SEM in mice.