不同方法制备金银花水提液对大鼠免疫功能影响的研究

目的探讨不同方法制备的金银花水提液对大鼠免疫功能的影响,为金银花的临床应用提供理论依据。方法分别采用水煎法和浸泡法制备金银花水提液,大鼠灌胃2周后,分别检测其巨噬细胞吞噬功能、淋巴细胞转化能力及TH1细胞分泌的IL-2、TNF-α、IFN-γ的mRNA表达情况。结果两种方法制备的金银花水提液均能改善机体的非特异性免疫功能,但只有水煎法制备的提取液能改善机体的细胞免疫功能。结论用水煎法制备金银花提取液改善机体免疫功能的功效优于浸泡法。

Effect of Flos Lonicera Extract Made by Different Methods on Immunologic Function of Rats

Objective To study the effect of Flos lonicera extract made by different methods on rats’ immunologic function,and to provide a theoretical basis for the clinical application of Flos lonicera. Methods After gastric irrigation of Flos lonicera extract by soaking and decocting for two weeks,the rats’ phagocytosis rate(%) of macrophage,transformation rate of lymphocyte and mRNA expression levels of IL-2,IFN-γ and TNF-α in TH1 were all detected. Results Phagocytosis rate(%) and phagocytosis index of macrophage were enhanced significantly by the drench of Flos lonicera extract made by two methods,but only Flos Lonicera extract made by decocting could improve cell mediate immunologic function of rats. Conclusions Flos lonicera extract made by decocting is superior to that by soaking in improving immunologic function.