胃腺癌组织中微淋巴管密度的检测及其临床病理意义

 目的探讨原发性胃腺癌中微淋巴管密度的临床病理意义。方法应用单克隆抗体D2-40检测原发性胃腺癌组织周边区和中心区及其正常胃组织中微淋巴管密度(LMVD),分析其与淋巴管浸润、淋巴结转移及其他临床病理参数之间的关系。结果胃腺癌周边区LMVD明显高于中心区LMVD(P<0.05)和正常胃组织LMVD(P<0.05),且淋巴管为功能性的。周边区LMVD与肿瘤的Ming分型、淋巴管浸润及淋巴结转移密切相关(P<0.05),而与患者的年龄、性别、肿瘤的分化程度及浸润程度无关(P>0.05)。中心区LMVD与这些临床病理参数之间均无相关性(P>0.05)。结论原发性胃腺癌癌周LMVD和淋巴管浸润、淋巴结转移密切相关,检测癌周LMVD是预测胃癌淋巴结转移的有效指标之一,有助于判断胃癌的预后。

Detection of Lymphatic Microvessel Density in Primary Gastric Adenocarcinoma and Its Clinicopathological Significance

Objective 　To investigate the clinicopathological significance of the lymphatic microvessel den2 sity (LMVD) in primary gast ric adenocarcinoma. Methods 　Monoclonal antibody D2240 was used immu2 nohistochemically to detect the lymphatic microvessel density(LMVD) in the int ra2 and peri2tumoral areas of the primary gast ric adenocarcinoma and normal gast ric tissue , and analysis the relationship among the lymphatic microvessel density (LMVD) , lymphatic invasion , lymph node metastasis and other clinico2 pathological parameters of the primary gast ric adenocarcinoma. Results 　LMVD of the peri2tumoral area was significantly higher than that of int ra2tumoral area and nomal tissue ( P < 0. 05) . LMVD of the peri2 tumoral area was significantly associated with Ming Classification , lymphatic invasion and lymph node metastasis ( P < 0. 05) , while less associated with age , sex , differentiation and invasion of the tumor ( P > 0. 05) . LMVD of the int ra2tumoral area was not correlated with these clinicopathological parameters ( P > 0. 05) . Conclusion 　LMVD of the peri2tumoral area was significantly associated with lymphatic invasion and lymph node metastasis , and detection of LMVD in peri2tumoral area can be used for prediction of lymph node metastasis.