尾静脉高压注射质粒DNA后体内表达的规律

目的研究小鼠尾静脉高压注射质粒DNA后，目的基因在体内分布表达的规律，以及该方法的转基因效率。方法将LacZ质粒按小鼠体重比稀释到一定的PBS中，经尾静脉快速高压注射导入小鼠体内，通过X-gal染色的方法在不同时间点观察目的基因在肝脏和其他脏器中的分布规律。结果含有CMV启动子的LaeZ裸质粒经小鼠尾静脉高压注射途径导入体内，24h后即开始表达，第3天出现表达高峰，1周后表达消失，基因转染率达5％-6％；用脂质体包裹质粒DNA后，再进行尾静脉高压注射，质粒在肝脏的表达效率可达18％-20％。结论应用尾静脉高压注射脂质体／质粒DNA复合物途径，目的基因在小鼠肝脏获得高效表达，该方法可以广泛应用于基因治疗的实验研究。

Plasmid DNA Distribution and Gene Expression Generated via Tail Vein Hydrodynamic Injection

Objective To investigate the regularity of gene expression and efficiency of gene transfection after tail vein injections of LacZ. Methods After diluting palsmid DNA in PBS solution according to the mouse weight, DNA was transferred via the tail vein by hydrodynamic injection. X-gal staining was performed to measure the LacZ gene expression in the liver, spleen, lung and kidney, Results The naked LacZ plasmid began to express on day 1 post injection, and reached the highest level at day 3, which was about 5%-6%, but diminished drastically by day 7. However, after encapsulated by liposome, the expression level of the same plasmid elevated to 18%-20%, via tail vein hydrodynamic injections. Conclusion High level gene expression in mouse liver can be achieved after tail vein hydrodynamic injections of liposome-encapsulated plasmid DNA, and this technique has great potential for a wide variety of gene therapy studies.