葛根素对牛视网膜血管内皮细胞舒缩因子的影响

目的：观察不同浓度葛根素对体外培养的牛视网膜血管内皮细胞舒缩因子的影响。

方法：取第8代牛视网膜血管内皮细胞用于实验。实验组中加入含不同浓度葛根素的培养液，对照组加入不含药物、无生长因子及血清的培养液。培养48h后用Western blotting 法测各组细胞eNOS及ET-1的表达。

结果：与阴性对照组比较，葛根素组eNOS的相对表达量均升高(P<0.01)，且随着药物浓度的增加eNOS的表达也增加。葛根素组ET-1的相对表达量均降低(P<0.01)，且随着药物浓度的增加ET-1表达下降。

结论：葛根素通过上调 eNOS的表达，下调ET-1的表达，调节血管一氧化氮内皮素(NO/ET)的比值，从而发挥松弛视网膜血管平滑肌的作用。

Effects of puerarin on the relaxing and contraction factors produced by bovine retina vascular endothelial cells

AIM: To observe the effects of puerarin of different concentrations on relaxing and contraction factors produced by cultured bovine retinal vascular endothelial cells(RVECs).

METHODS: The eighth generation of bovine RVECs was used for the research. The RVECs were cultivated in nutrient solution with different concentrations of puerarin in experimental group and in nutrient solution without drug, growth factor and serum in the control group. Expressions of eNOS and ET-1 in each group were measured by Western blotting method after 48h.

RESULTS:Compared with the negative comparison group, relative expression of eNOS in puerarin group increased(P<0.01), and eNOS expression also enhanced with the increase of the concentration of drugs; the ET-1 relative expressions of puerarin group decreased(P<0.01), and ET-1 expression also decreased with the increase of the concentration of drugs.

CONCLUSION:By up-regulating eNOS expression, down-regulating the ET-1 expression and regulating vascular nitric oxide endothelin(NO/ET)ratio, puerarin can relax retinal vascular smooth muscle.