多西紫杉醇诱导前列腺癌PC3细胞凋亡的蛋白质组学研究

目的 比较多西紫杉醇诱导激素非依赖性前列腺癌PC3细胞凋亡前后的差异表达蛋白。方法 采用磺酰罗丹明B(SRB)法检测不同浓度多西紫杉醇对前列腺癌PC3细胞增殖的抑制作用；应用胶内差异凝胶电泳对PC3细胞凋亡前后蛋白质进行双向凝胶电泳图谱分析，分离并筛选鉴定相关差异蛋白。结果 半数抑制浓度IC50为20nmol/L。双向凝胶电泳图谱发现表达差异较为明显的18个蛋白质位点，其中8个蛋白表达上调，10个蛋白表达下调。结论 本研究发现的部分差异蛋白可能参与激素非依赖性前列腺癌对紫杉醇类药物的耐药作用。

A proteomics study of docetaxel-induced apoptosis in prostate cancer PC3 cells

Objective To investigate the preliminary molecule mechanism of docetaxel resistance in prostate cancer cells by comparing the differential protein expressions before and after docetaxel-induced apoptosis in androgen independent prostate cancer PC3 cells. Methods The sulforhodamine B method was used to detect the inhibition effect of different concentrations of docetaxel on the proliferation of PC3 cells. Differential gel electrophoresis (DIGE) was adopted to separate and identify the differential proteins before and after docetaxel-induced apoptosis. Results The median inhibitory concentration IC50 was 20nmol/L. Eighteen significant differential protein spots were defined by DIGE. Eight proteins were up-regulated while ten proteins were down-regulated. Conclusion Eighteen significant differential protein spots were identified by using the proteomics method. It was believded that various differential proteins might be involved in the docetaxel resistance in androgen independent prostate cancer therapy.