一起诺沃克病毒引起的急性胃肠炎爆发的分子流行病学研究

目的 查明2005年深圳市一起急性胃肠炎的病原体,为制定控制疾病流行策略及指导临床合理用药提供依据.方法 收集该次腹泻患者及相关人员的粪便标本15份,采用逆转录-聚合酶链反应(RT-PCR)和实时荧光PCR方法检测诺沃克病毒核糖核酸(RNA).结果 15份标本中,RT-PCR及实时荧光PCR方法均检测出10例诺沃克病毒阳性,阳性率为56.7%;2种方法的符合率为100%.同时对其中的10份RT-PCR阳性标本进行脱氧核糖核酸(DNA)序列测定,并进行同源性分析和进化分析.结论 该次群体性胃肠炎是由诺沃克病毒G2型感染引起;10份阳性标本中9株的核苷酸同源性为100%,另外1株与其他9株的同源性为99.8%;本次流行的毒株(05-53-61及05-63)与日本东京的SaitamaU201及SaitamaU18亲缘关系较近,核苷酸同源性分别为96%及95%,而与1997年深圳本地株97-11及97-30亲缘关系则较远,核苷酸同源性分别为76.4%及76.7%.

Molecular epidemiology research on an outbreak of acute gastroenteritis by Norwalk-like virus

Objective This study was conducted to investigate the pathogen of an outbreak of acute gastroenteritis in 2005, and to provide scientific data for the control of infectious diseases and instructions on clinical therapy. Methods 15 stool samples from diarrhea patients and related people were collected, and RT-PCR and real-time PCR were used to detect the virus RNA. Results 10 out of 15 samples had been confirmed for tested positive (66.7%), the coincidence rate between of the two methods was 100%. The DNA sequence was tested in positive samples were partially sequencetod and analyzmakeing for homologicaly and evolutional analysis. Conclusion The outbreak was caused by Norwalk-like virus G2 type, and 9 out of 10 cases was 100% homological, and the 1 left is 99.8% homological to the others. The epidemic strain (05-53-61 and 05-63) was 96% and 95% homologicaly to Norwalk virus strain SaitamaU201 and SaitamaU18, respectively. It was 76.4% and 76.7% homologicaly to the strain 97-11 and 97-30 in Shenzhen.