双波长HPLC同时测定生地黄中6种化合物的含量

目的 采用双波长HPLC同时测定生地黄中6种化合物（梓醇、地黄苷D、地黄苷A、益母草苷、毛蕊花糖苷、异类叶升麻苷）的含量。方法 菲罗门Neptune C₁₈色谱柱（4.6 mm×250 mm，5μm）；流动相：乙腈-0.1%磷酸溶液，梯度洗脱；流速：1.0 mL·min^-1；柱温30℃；检测波长203 nm（梓醇、地黄苷D、地黄苷A、益母草苷），334 nm（毛蕊花糖苷、异类叶升麻苷）。结果 6种化合物在各自的范围内呈现良好的线性关系（r>0.9990），3个浓度水平回收率96.33%~98.55%，RSD为0.97%~1.45%。结论 该法简单快捷、稳定可靠，准确率高，重复性好，可用于生地黄质量评价。

Simultaneous determination of 6 compounds in Rehmanniae Radix by dual-wavelength HPLC

OBJECTIVE To establish a dual-wave length HPLC method for the determination of 6 components in Rehmanniae Radix (including catalpol, rehmannioside D, rehmannioside A, leonuride, acteoside and isoacteoside). METHODS Neptune C₁₈ column(4.6 mm×250 mm, 5 μm) was selected for separation with acetonitrile-0.1% phosphoric acid solution as the mobile phase for gradient elution, the flow rate was 1.0 mL·min^-1, the column temperature was maintained at 30 ℃ and the detection wavelength was set at 203 nm for catalpol, rehmannioside D, rehmannioside A and leonuride, 334 nm for acteoside and isoacteoside. RESULTS Six components showed good linear relationships(r>0.9990) in their own ranges. The average recoveries at three levels ranged from 96.33% to 98.55% with RSD of 0.97% to 1.45%. CONCLUSION This method is simple, accurate, reliable, stable and reproducible, which can be used for the quality control of Rehmanniae Radix.