CD44+/CD24-表型与BRCA1及basal-like乳腺癌的相关性

 目的：探讨CD44⁺/CD24^-表型与BRCA1及basal-like乳腺癌的相关性。方法：收集经病理诊断为乳腺癌患者的手术切除石蜡标本共217例，根据免疫学标志物把217例乳腺癌划分为5类分子亚型：basal-like型、luminal A型、luminal B 型、Her2过表达型和normal breast-like型。应用免疫组织化学检测CK5/6、BRCA1和CD44/CD24双染的情况，分析CD44⁺/CD24^-表型与basal-like乳腺癌及BRCA1相关性乳腺癌的关系。结果：217例乳腺癌标本例中,luminal A型130例、luminal B型15例、HER2过表达型21例、basal-like型29例、Normal breast-like型22例。BRCA1相关性乳腺癌57例。basal-like乳腺癌组织中BRCA1缺失率86%（25/29），明显高于乳腺癌其他分子亚型(P<0.001)。basal-like乳腺癌组织中含CD44⁺/CD24^-肿瘤细胞者20例 (20/29,69%)，明显高于乳腺癌其他分子亚型(P=0.003);BRCA1相关性乳腺癌中含CD44⁺/CD24^- 肿瘤细胞者53例(53/57,93%)。结论：BRCA1基因突变与basal-like乳腺癌有关;CD44⁺/CD24^-干细胞表型主要存在于basal-like乳腺癌及BRCA1相关性乳腺癌中。

Relationship between CD44+/CD24- Phenotype and BRCA1,basal-like Breast Cancer

Objective：To study the expression of CD44⁺/CD24^- phenotype and its association with the basal-like breast cancer and BRCA1 in breast cancer．Methods：Using immunostaining ER,PR,HER2 and CK5/6 to classify the material into five tumor subgroups: basal-like subgroup,Luminal A subgroup,Luminal B subgroup,Her2 over- expressing subgroup and normal breast-like subgroup.Double-staining immunohistochemistry (IHC) was applied to the detection of CD44⁺/CD24^-cells and single-staining(IHC) for CK5/6,BRCA1.The correlations of the expression of the CD44⁺/CD24^- to basal-like and BRCA1-associated breast cancer were analyzed.Results ：In 217 cases of breast cancer, Luminal A subgroup: 130 cases,Luminal B subgroup: 15 cases,Her2 over-expressing subgroup: 21 cases,basal-like subgroup:29 cases,Normal breast-like subgroup:22 cases.BRCA1-associated breast cancer:57 cases.The expression rates of BRCA1 was low in basal-like breast cancer, which was significantly different from the other subgroups (P<0.001).The expression rates of CD44⁺/CD24^- phenotype was high in basal-like breast cancer (20/29,69%)and BRCA1-associated breast cancer(53/57,93%), which was significantly different from the other subgroups (P=0.003).Conclusion：The mutation of BRCA1 is association with basal-like breast cancer;and the CD44⁺/CD24^- breast cancer stem cells in tumor tissue is association with BRCA1-associated breast cancer and basal-like breast cancer.